Alves Décio Luis, Lima Sônia Maria Rolim Rosa, da Silva Célia Regina, Galvão Maria Antonieta Longo, Shanaider Alberto, de Almeida Prado Roberto Adelino, Aoki Tsutomu
Obstetrics and Ginecology Department of the Santa Casa de São Paulo, Faculty of Medical Sciences, São Paulo, Brazil.
Maturitas. 2008 Dec 20;61(4):364-70. doi: 10.1016/j.maturitas.2008.08.006. Epub 2008 Dec 17.
The aim of this study was to evaluate the effects of Trifolium pratense and Cimicifuga racemosa upon the endometrium of castrated female Wistar rats, comparing these results with a placebo and estradiol valerate.
Thirty-two adult castrated female Wistar rats were divided into four groups (eight rats per group) that receiving either tap water, estradiol valerate, isoflavones from T. pratense or deoxyactein from C. racemosa daily. The doses used were equivalent to normal doses used in humans. The results were analyzed by endometrial histology and the expression of alpha-estrogen receptor and protein Ki67. Both alpha-receptor and Ki67 expressions were determined by immunohistochemical and morphometric analysis.
Endometrium histology stayed atrophic with both herbal extracts, but T. pratense supplementation increased the expression of alpha-estrogen receptors when compared to the placebo group, without protein Ki67 expression enhancement. Both herbal extracts presented a lower Ki67 expression when compared to the placebo group.
T. pratense presented alpha-estrogen receptor stimulation in the endometrium without increasing cell proliferation. Both herbal extracts reduced endometrial proliferation in comparison to the placebo group.
本研究旨在评估红车轴草和总状升麻对去势雌性Wistar大鼠子宫内膜的影响，并将这些结果与安慰剂和戊酸雌二醇进行比较。
将32只成年去势雌性Wistar大鼠分为四组（每组8只），分别每日给予自来水、戊酸雌二醇、红车轴草中的异黄酮或总状升麻中的脱氧阿替因。所用剂量相当于人类正常使用剂量。通过子宫内膜组织学以及α-雌激素受体和蛋白Ki67的表达来分析结果。α-受体和Ki67的表达均通过免疫组织化学和形态计量学分析来确定。
两种草药提取物处理后的子宫内膜组织学均呈萎缩状态，但与安慰剂组相比，补充红车轴草可增加α-雌激素受体的表达，而未增强蛋白Ki67的表达。与安慰剂组相比，两种草药提取物的Ki67表达均较低。
红车轴草可在不增加细胞增殖的情况下刺激子宫内膜中的α-雌激素受体。与安慰剂组相比，两种草药提取物均降低了子宫内膜增殖。
