Morgan Philip E, Sturgess Allan D, Davies Michael J
The Heart Research Institute, Camperdown, Sydney, NSW, Australia.
Free Radic Res. 2009 Feb;43(2):117-27. doi: 10.1080/10715760802623896.
Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
自身免疫性疾病系统性红斑狼疮（SLE）患者的血清蛋白氧化水平此前已被证明：（a）在单个时间点升高；（b）与疾病活动相关。本研究通过分析21例SLE患者和9例对照在长达38个月的时间内采集的多个血清样本，调查这种升高是否为慢性现象。与对照组相比，疾病活动稳定或变化的SLE患者的蛋白质巯基长期减少，而蛋白质结合羰基和甘氨酸则长期增加。羰基分布的二维凝胶分析表明，白蛋白和免疫球蛋白受到了特别的影响。在疾病活动稳定的SLE患者中，较高的长期蛋白质氧化与较高的长期疾病活动相关。疾病活动变化的SLE患者在蛋白质氧化与疾病活动标志物之间表现出不同的相关性。这些结果进一步支持了氧化应激在SLE发病机制中的作用。
