Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Cno. de Vera s/n, 46022 Valencia, Spain.
Departamento de Ingeniería Biomédica, Universidad Iberoamericana, Prolongación Paseo de la Reforma 880, Lomas de Santa Fe, Ciudad de México 01219, Mexico.
Int J Mol Sci. 2023 Jun 19;24(12):10340. doi: 10.3390/ijms241210340.
Controlled drug release systems are the subject of many investigations to achieve the therapeutic effect of drugs. They have numerous advantages, such as localized effects, lower side effects, and less onset of action. Among drug-delivery systems, electrospinning is a versatile and cost-effective method for biomedical applications. Furthermore, electrospun nanofibers are promising as drug carrier candidates due to their properties that mimic the extracellular matrix. In this work, electrospun fibers were made of Poly-L-lactic acid (PLA), one of the most widely tested materials, which has excellent biocompatible and biodegradable properties. A curcuminoid, bisdemethoxycurcumin (BDMC) was added in order to complete the drug delivery system. The PLA/BDMC membranes were characterized, and biological characteristics were examined in vitro. The results show that the average fiber diameter was reduced with the drug, which was mainly released during the first 24 h by a diffusion mechanism. It was seen that the use of our membranes loaded with BDMC enhanced the rate of proliferation in Schwann cells, the main peripheral neuroglial cells, and modulated inflammation by reducing NLRP3 inflammasome activation. Considering the results, the prepared PLA/BDMC membranes hold great potential for being used in tissue engineering applications.
控释药物释放系统是许多旨在实现药物治疗效果的研究的主题。它们具有许多优点，例如局部作用、较低的副作用和较少的作用开始时间。在药物输送系统中，静电纺丝是一种用于生物医学应用的多功能且具有成本效益的方法。此外，由于其模拟细胞外基质的特性，静电纺纳米纤维作为药物载体候选物具有广阔的前景。在这项工作中，使用聚乳酸（PLA）制成了电纺纤维，PLA 是经过最广泛测试的材料之一，具有极好的生物相容性和可生物降解性。添加了姜黄素类化合物双去甲氧基姜黄素（BDMC）以完成药物输送系统。对 PLA/BDMC 膜进行了表征，并在体外检查了其生物学特性。结果表明，纤维的平均直径随药物的加入而减小，药物主要通过扩散机制在前 24 小时内释放。可以看出，使用我们负载 BDMC 的膜增强了雪旺细胞（主要的周围神经胶质细胞）的增殖速度，并通过减少 NLRP3 炎性小体的激活来调节炎症。考虑到这些结果，制备的 PLA/BDMC 膜在组织工程应用中具有很大的应用潜力。
