Voller J, Donek A, Cendelń J, Korelusová I, Vozeh F
Charles University in Prague, Faculty of Medicine in Pilsen, Department of Pathophysiology, Czech Republic.
Prague Med Rep. 2008;109(1):32-9.
Dopaminergic neurotransmitter system plays a role in many neural functions, for instance in the motor system. Dopamine also influences cognitive functions, motivation and behaviour. In this study, the effect of D1-like receptors blocker SCH 23390 on spontaneous motor activity, motor functions and spatial learning in adult wild type mice derived from the strain B6CBA was investigated. The animals of one group were administered with D1-like receptors blocker SCH 23390 while the mice from the other group were treated with the saline solution 20 minutes before experiments. Spontaneous motor activity was examined in the open field. Motor functions were examined using horizontal wire, ladder and rotarod. Spatial learning was tested in the Morris water maze. D1-like receptors inhibition significantly decreased spontaneous motor activity. In the horizontal wire test, the mice treated with SCH 23390 manifested significantly better results than control animals. No significant differences between experimental animals and controls were found in the ladder test. On the rotarod, the animals with D1-like receptors inhibition reached significantly shorter latencies than the animals treated with the saline solution. D1-like receptors blockade led to significant impairment of performance in experimental animals in the Morris water maze. Spontaneous motor activity and motor skills were influenced by evoked hypokinesia. Meanwhile in the horizontal wire test this effect seemed to be advantageous, in the rotarod test it meant a definite handicap because this test requires good motor coordination and activity. The results gained in the Morris water maze indicated that not only hypokinesia played some role there but also spatial learning and perhaps motivation were affected. The study confirmed that D1-like receptors inhibition has effects on both motor and cognitive functions in mice. However the motor effects are not the main cause of the failing in the spatial learning test.
多巴胺能神经递质系统在许多神经功能中发挥作用，例如在运动系统中。多巴胺还会影响认知功能、动机和行为。在本研究中，研究了D1样受体阻滞剂SCH 23390对源自B6CBA品系的成年野生型小鼠自发运动活动、运动功能和空间学习的影响。一组动物在实验前20分钟给予D1样受体阻滞剂SCH 23390，而另一组小鼠用生理盐水处理。在旷场中检测自发运动活动。使用水平钢丝、梯子和转棒来检测运动功能。在莫里斯水迷宫中测试空间学习能力。抑制D1样受体会显著降低自发运动活动。在水平钢丝测试中，用SCH 23390处理的小鼠表现出比对照动物明显更好的结果。在梯子测试中，实验动物和对照之间未发现显著差异。在转棒上，抑制D1样受体的动物达到潜伏期的时间明显短于用生理盐水处理的动物。在莫里斯水迷宫中，D1样受体阻断导致实验动物的表现显著受损。诱发的运动减退会影响自发运动活动和运动技能。同时，在水平钢丝测试中这种影响似乎是有利的，而在转棒测试中这意味着明显的不利，因为该测试需要良好的运动协调性和活动能力。在莫里斯水迷宫中获得的结果表明，不仅运动减退在其中起了一定作用，而且空间学习以及可能的动机也受到了影响。该研究证实，抑制D1样受体对小鼠的运动和认知功能均有影响。然而，运动效应并非空间学习测试失败的主要原因。
