Yu Xiao-Jia, Zhan Rui, Huang Hong, Ding Guang-Hong
Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China.
Zhen Ci Yan Jiu. 2008 Oct;33(5):310-5.
To observe the difference of signaling afferent mechanism of acupoints analgesic effect between manual acupuncture (MA) and electroacupuncture (EA) of "Zusanli" (ST 36) in order to analyze the initiating mechanism of these two commonly used clinic acupuncture methods.
A total of 64 SD rats were randomized into control, model, MA, EA, collagenase (type- I)-pretreatment (CP) + MA, CP + EA, disodium cromoglicate-pretreatment (DSCP) + MA and DSCP + EA groups with 8 cases in each. Arthritis model was established by intra-joint injection of complete Freunds' agent. MA(rotation, 20 min), and EA (21 min) were applied to bilateral "Zusanli" (ST 36) respectively in different groups. Pain threshold (PT, paw withdraw latency) was detected with 336 GT PAW/TAIL STIMULATOR and degranulated mast cells were counted under microscope separately.
Compared with normal control group, PT values of model, MA, EA, CP + MA, CP + EA, DSCP + MA and DSCP + EA groups lowered significantly before treatment (P < 0.05). Comparison of the same one group showed that after the treatment, PT values of MA and EA groups increased considerably (P < 0.05). PT changing rates of CP + MA and DSCP + MA were significantly lower than that of MA group (P < 0.05), suggesting disappearance of MA analgesia (not EA analgesia) after lesion of collagen protein by CP in ST36 area. In comparison with control group, the degranulation ratios (DGR) of mast cells in MA and EA groups increased significantly (P < 0.05); while compared with MA and EA groups separately, DGR of the corresponding CP + MA and DSCP + MA, and CP + EA and DSCP + EA groups decreased markedly (P < 0.05), displaying an inhibition of mast cell degranulation following CP and DSCP in ST36 area.
Both MA and EA can induce analgesia and concomitant increase of DGR of mast cells. The collagen fibers may contribute to MA initiated analgesic effect while the peripheral nerve receptors are probably involved in EA analgesia in rat.
观察手针（MA）与电针（EA）“足三里”（ST 36）穴位镇痛效应的信号传入机制差异，以分析这两种临床常用针刺方法的起始机制。
将64只SD大鼠随机分为对照组、模型组、MA组、EA组、Ⅰ型胶原酶预处理（CP）+MA组、CP+EA组、色甘酸钠预处理（DSCP）+MA组和DSCP+EA组，每组8只。通过关节内注射完全弗氏佐剂建立关节炎模型。不同组分别对双侧“足三里”（ST 36）进行MA（捻转，20分钟）和EA（21分钟）治疗。用336 GT PAW/TAIL刺激器检测痛阈（PT，爪退缩潜伏期），并在显微镜下分别计数脱颗粒肥大细胞。
与正常对照组相比，模型组、MA组、EA组、CP+MA组、CP+EA组、DSCP+MA组和DSCP+EA组治疗前PT值显著降低（P<0.05）。同一组比较显示，治疗后MA组和EA组PT值显著升高（P<0.05）。CP+MA组和DSCP+MA组的PT变化率显著低于MA组（P<0.05），提示在ST36区用CP破坏胶原蛋白后MA镇痛作用消失（而非EA镇痛作用）。与对照组相比，MA组和EA组肥大细胞脱颗粒率（DGR）显著升高（P<0.05）；而分别与MA组和EA组相比，相应的CP+MA组和DSCP+MA组以及CP+EA组和DSCP+EA组的DGR显著降低（P<0.05），显示在ST36区CP和DSCP后肥大细胞脱颗粒受到抑制。
MA和EA均可诱导镇痛并伴随肥大细胞DGR增加。胶原纤维可能有助于MA引发的镇痛作用，而大鼠EA镇痛可能涉及外周神经受体。
