Shanghai Institute of Acupuncture-Moxibustion and Meridians, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.
World J Gastroenterol. 2011 Aug 28;17(32):3733-8. doi: 10.3748/wjg.v17.i32.3733.
To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis.
A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxibustion group, moxibustion plus disodium cromoglycate (M + DC) group and moxibustion plus normal saline (M + NS) group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M + DC and M + NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxylin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas.
An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group (45.91 ± 11.41 vs 32.58 ± 8.28, P < 0.05). After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed.
Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint.
观察艾灸天枢穴对三硝基苯磺酸（TNBS）诱导的结肠炎大鼠肠组织肥大细胞（MC）形态和功能的影响。
53 只雄性 SD 大鼠随机分为正常组和实验组。实验组采用 TNBS 诱导结肠炎模型，将大鼠随机分为模型组、艾灸组、艾灸加色甘酸钠（M+DC）组和艾灸加生理盐水（M+NS）组。艾灸组大鼠在双侧天枢穴进行悬灸，每次 10 min，每日 1 次，共 7 d。M+DC 组和 M+NS 组大鼠分别在双侧天枢穴预处理色甘酸钠和生理盐水，然后同时给予与艾灸组相同的处理。采用苏木精-伊红（HE）染色法观察结肠组织学变化，甲苯胺蓝改良法观察天枢穴区 MC 脱颗粒情况。
艾灸组大鼠结肠损伤明显改善，天枢穴区 MC 脱颗粒率明显高于模型组（45.91±11.41 比 32.58±8.28，P<0.05）。用色甘酸钠抑制天枢穴区 MC 脱颗粒后，结肠组织损伤未见改善。
艾灸通过增加局部 MC 脱颗粒率，对 TNBS 诱导的结肠炎大鼠受损结肠黏膜愈合发挥作用，但对天枢穴区 MC 形态无明显影响。
