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在胶原酶诱导的骨关节炎大鼠模型中，电针诱导的镇痛作用由5-羟色胺1、5-羟色胺3受体和毒蕈碱型胆碱能受体介导。

The electroacupuncture-induced analgesic effect mediated by 5-HT1, 5-HT3 receptor and muscarinic cholinergic receptors in rat model of collagenase-induced osteoarthritis.

作者信息

Seo Byung-Kwan, Sung Won-Suk, Park Yeon-Cheol, Baek Yong-Hyeon

机构信息

Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Korea.

出版信息

BMC Complement Altern Med. 2016 Jul 13;16:212. doi: 10.1186/s12906-016-1204-z.

DOI:10.1186/s12906-016-1204-z
PMID:27411565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4943008/
Abstract

BACKGROUND

Osteoarthritis (OA) is an degenerative disease characterized by chronic joint pain. Complementary and alternative treatment such as acupuncture have been utilized to alleviate pain. The objective of this study was to investigate the analgesic mechanisms of electroacupuncture (EA) in the collagenase-induced osteoarthritis (CIOA) rat model.

METHODS

Four weeks after inducing CIOA by injecting collagenase solution into the left knee of 5-week-old male Sprague-Dawley rats, 2 Hz and 100 Hz EA on Zusanli (ST 36) was performed. The analgesic effect of EA was evaluated by the tail flick latency (TFL) and paw pressure threshold (PPT) tests. To investigate the analgesic mechanism, serotonergic and muscarinic cholinergic receptor agonists and antagonists were injected 20 min prior to EA and the resultant changes were evaluated by the TFL and PPT tests.

RESULTS

EA on Zusanli (ST 36) demonstrated an analgesic effect in the CIOA rat model. The 2 Hz EA treatment showed a significantly greater analgesic effect than the 100 Hz treatment. The analgesic effect of 2 Hz EA was not strengthened by 5-HT1, 5-HT2, 5-HT3, and muscarinic cholinergic receptor agonist pretreatment, was blocked by 5-HT1, 5-HT3, and muscarinic cholinergic receptor antagonist pretreatment, but not blocked by 5-HT2 receptor antagonist pretreatment.

CONCLUSIONS

In the CIOA rat model, EA on Zusanli (ST 36) exhibited analgesic effects, and 2 Hz EA resulted in a significantly greater analgesic effect than 100 Hz EA. The analgesic effect of 2 Hz EA was reduced by pretreatment of 5-HT1 receptor, 5-HT3 receptor and muscarinic cholinergic receptor antagonists.

摘要

背景

骨关节炎（OA）是一种以慢性关节疼痛为特征的退行性疾病。针灸等补充和替代疗法已被用于缓解疼痛。本研究的目的是探讨电针（EA）在胶原酶诱导的骨关节炎（CIOA）大鼠模型中的镇痛机制。

方法

将胶原酶溶液注射到5周龄雄性Sprague-Dawley大鼠的左膝诱导CIOA四周后，在足三里（ST 36）进行2Hz和100Hz的电针治疗。通过甩尾潜伏期（TFL）和爪压力阈值（PPT）测试评估电针的镇痛效果。为了研究镇痛机制，在电针前20分钟注射血清素能和毒蕈碱胆碱能受体激动剂和拮抗剂，并通过TFL和PPT测试评估由此产生的变化。

结果

足三里（ST 36）电针在CIOA大鼠模型中显示出镇痛作用。2Hz电针治疗显示出比100Hz治疗明显更强的镇痛效果。5-HT1、5-HT2、5-HT3和毒蕈碱胆碱能受体激动剂预处理未增强2Hz电针的镇痛效果，5-HT1、5-HT3和毒蕈碱胆碱能受体拮抗剂预处理阻断了该效果，但5-HT2受体拮抗剂预处理未阻断。

结论

在CIOA大鼠模型中，足三里（ST 36）电针表现出镇痛作用，且2Hz电针产生的镇痛效果明显强于100Hz电针。5-HT1受体、5-HT3受体和毒蕈碱胆碱能受体拮抗剂预处理降低了2Hz电针的镇痛效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/4595433602bd/12906_2016_1204_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/a5abc41435e6/12906_2016_1204_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/cb1a49d6d71a/12906_2016_1204_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/066a0c21d42d/12906_2016_1204_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/ebdba8aca900/12906_2016_1204_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/ce74fbe51066/12906_2016_1204_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/4595433602bd/12906_2016_1204_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/a5abc41435e6/12906_2016_1204_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/cb1a49d6d71a/12906_2016_1204_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/066a0c21d42d/12906_2016_1204_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/ebdba8aca900/12906_2016_1204_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/ce74fbe51066/12906_2016_1204_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c062/4943008/4595433602bd/12906_2016_1204_Fig6_HTML.jpg

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