Kapur P, Pereira B M J, Wuttke W, Jarry H
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Phytomedicine. 2009 Jun;16(6-7):679-82. doi: 10.1016/j.phymed.2008.10.005. Epub 2008 Dec 20.
Recently, Tinospora cordifolia (TC) was shown to affect prostate growth in rats. It is not known whether this is a direct effect of TC or whether it is induced by altered hormone release. To investigate the actions of TC on the prostate, human LNCaP cells were exposed to an ethanolic extract of TC.
LNCaP cells were incubated with the test substances for 48 h. Proliferation was measured by MTT test and prostate-specific antigen (PSA) secretion was determined with ELISA.
TC showed a dose-dependent stimulation of proliferation of LNCaP cells. Co-incubation with the anti-androgen flutamide (FLU) reversed the TC-induced stimulation of PSA secretion.
The reference compound dihydrotestosterone (DHT) caused a significant increase of growth of LNCaP cells. Similarly, TC stimulated proliferation of these prostate cells. The anti-androgen FLU reversed the increase of PSA release caused by either DHT or TC. Thus, we suggest that TC may contain androgenic compounds, which appear to act via androgen receptor (AR).
最近，显示出心叶青牛胆（TC）会影响大鼠前列腺的生长。尚不清楚这是TC的直接作用，还是由激素释放改变所诱导。为了研究TC对前列腺的作用，将人LNCaP细胞暴露于TC的乙醇提取物中。
将LNCaP细胞与测试物质孵育48小时。通过MTT试验测量增殖，并使用ELISA测定前列腺特异性抗原（PSA）分泌。
TC显示出对LNCaP细胞增殖的剂量依赖性刺激。与抗雄激素氟他胺（FLU）共同孵育可逆转TC诱导的PSA分泌刺激。
参考化合物二氢睾酮（DHT）导致LNCaP细胞生长显著增加。同样，TC刺激了这些前列腺细胞的增殖。抗雄激素FLU逆转了由DHT或TC引起的PSA释放增加。因此，我们认为TC可能含有雄激素化合物，其似乎通过雄激素受体（AR）起作用。
