一种以喹啉敏化剂为中心的新型镧系螯合物及其在用于时间分辨荧光共振能量转移分析的镍-次氮基三乙酸（Ni-NTA）磁珠上进行蛋白质标记的应用。
A new quinoline sensitizer-centered lanthanide chelate and its use for protein labling on Ni-NTA beads for TR LRET assays.
作者信息
Kim Sung Hoon, Ge Pinghua, Katzenellenbogen John A
机构信息
Department of Chemistry of University of Illinois, 600 South Mathews Avenue, Urbana, IL 61801, USA.
出版信息
Chem Commun (Camb). 2009 Jan 8(2):183-5. doi: 10.1039/b814980a. Epub 2008 Nov 18.
Abstract
A quinoline sensitizer-centered lanthanide chelate system of novel design for TR-LRET was prepared; it exhibited high labelling efficiency with a his-tagged protein (ERalpha-LBD) on the Ni-NTA beads, using a mixed metal chelate protocol, and it functioned well in TR-LRET protein-protein interaction assays.
摘要
制备了一种用于时间分辨荧光共振能量转移（TR-LRET）的新型设计的以喹啉敏化剂为中心的镧系螯合物体系；使用混合金属螯合方案，它在镍-氮三乙酸（Ni-NTA）磁珠上对带有组氨酸标签的蛋白质（雌激素受体α配体结合域，ERalpha-LBD）表现出高标记效率，并且在TR-LRET蛋白质-蛋白质相互作用测定中功能良好。
相似文献
1
A new quinoline sensitizer-centered lanthanide chelate and its use for protein labling on Ni-NTA beads for TR LRET assays.一种以喹啉敏化剂为中心的新型镧系螯合物及其在用于时间分辨荧光共振能量转移分析的镍-次氮基三乙酸（Ni-NTA）磁珠上进行蛋白质标记的应用。
Chem Commun (Camb). 2009 Jan 8(2):183-5. doi: 10.1039/b814980a. Epub 2008 Nov 18.
2
Development and characterization of Ni-NTA-bearing microspheres.含镍-氮三乙酸微球的制备与表征
Cytometry. 2002 Jul 1;48(3):136-45. doi: 10.1002/cyto.10124.
3
High-efficiency Ni-NTA/PAA magnetic beads with specific separation on His-tagged protein.高效 Ni-NTA/PAA 磁珠对 His 标记蛋白具有特异性分离。
IET Nanobiotechnol. 2020 Feb;14(1):67-72. doi: 10.1049/iet-nbt.2019.0271.
4
Reversible immobilization of engineered molecules by Ni-NTA chelators.通过镍-次氮基三乙酸螯合剂对工程分子进行可逆固定。
Bioelectrochemistry. 2004 Jun;63(1-2):271-5. doi: 10.1016/j.bioelechem.2003.10.024.
5
Dual-mode fluorophore-doped nickel nitrilotriacetic acid-modified silica nanoparticles combine histidine-tagged protein purification with site-specific fluorophore labeling.双模式荧光团掺杂的次氮基三乙酸镍修饰的二氧化硅纳米颗粒将组氨酸标签蛋白纯化与位点特异性荧光团标记相结合。
J Am Chem Soc. 2007 Oct 31;129(43):13254-64. doi: 10.1021/ja074443f. Epub 2007 Oct 2.
6
Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions.六组氨酸标签特异性光学探针用于蛋白质及其相互作用分析。
Anal Biochem. 2010 Apr 15;399(2):237-45. doi: 10.1016/j.ab.2009.12.028. Epub 2009 Dec 28.
7
Silicatein conjugation inside nanoconfined geometries through immobilized NTA-Ni(II) chelates.通过固定化 NTA-Ni(II)螯合物在纳米受限几何结构内进行硅蛋白的缀合。
Chem Commun (Camb). 2013 Mar 18;49(22):2210-2. doi: 10.1039/c3cc38605h.
8
Compact and versatile nickel-nitrilotriacetate-modified quantum dots for protein imaging and Förster resonance energy transfer based assay.用于蛋白质成像和基于Förster 共振能量转移的分析的紧凑型多功能镍-氮基三乙酸酯修饰量子点。
Langmuir. 2010 May 18;26(10):7327-33. doi: 10.1021/la9041887.
9
Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on ni-nitrilotriacetic acid surfaces.具有高亲和力结合能力的双六组氨酸标签，用于在镍-次氮基三乙酸表面固定、纯化和检测蛋白质。
Anal Chem. 2006 May 1;78(9):3072-9. doi: 10.1021/ac060184l.
10
Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine.使用金属螯合半胱氨酸对多组氨酸序列进行位点特异性荧光标记。
Chem Biol Drug Des. 2007 Jan;69(1):31-40. doi: 10.1111/j.1747-0285.2007.00463.x.
引用本文的文献
1
Tailoring Nanomaterial Cross-Linkers through Lanthanide-Ligand Pairs: Guidance for Fine-Tuning the Structures and Properties of Luminescent Nanocomposite Hydrogels.通过镧系元素-配体对定制纳米材料交联剂：微调发光纳米复合水凝胶结构与性能的指南
Inorg Chem. 2025 May 5;64(17):8601-8619. doi: 10.1021/acs.inorgchem.5c00130. Epub 2025 Apr 23.
2
Biosensors Based on the Binding Events of Nitrilotriacetic Acid-Metal Complexes.基于氮川三乙酸-金属配合物结合事件的生物传感器。
Biosensors (Basel). 2023 Apr 28;13(5):507. doi: 10.3390/bios13050507.
3
Efficient route to pre-organized and linear polyaminopolycarboxylates: Cy-TTHA, Cy-DTPA and mono/di- reactive, -butyl protected TTHA/Cy-TTHA.通往预组织和线性聚氨基多羧酸盐的有效途径：Cy-TTHA、Cy-DTPA以及单/双反应性、丁基保护的TTHA/Cy-TTHA。
Tetrahedron Lett. 2017 Apr 12;58(15):1441-1444. doi: 10.1016/j.tetlet.2017.02.056. Epub 2017 Feb 20.
本文引用的文献
1
Time-resolved fluorescence resonance energy transfer kinase assays using physiological protein substrates: applications of terbium-fluorescein and terbium-green fluorescent protein fluorescence resonance energy transfer pairs.使用生理蛋白质底物的时间分辨荧光共振能量转移激酶测定：铽-荧光素和铽-绿色荧光蛋白荧光共振能量转移对的应用
Anal Biochem. 2006 Sep 1;356(1):108-16. doi: 10.1016/j.ab.2006.05.017. Epub 2006 Jun 9.
2
Improving lanthanide-based resonance energy transfer detection by increasing donor-acceptor distances.通过增加供体-受体距离来改进基于镧系元素的共振能量转移检测。
J Biomol Screen. 2006 Jun;11(4):439-43. doi: 10.1177/1087057106287142. Epub 2006 Apr 28.
3
Modulation of luminescence intensity of lanthanide complexes by photoinduced electron transfer and its application to a long-lived protease probe.通过光诱导电子转移调控镧系配合物的发光强度及其在长寿命蛋白酶探针中的应用。
J Am Chem Soc. 2006 May 31;128(21):6938-46. doi: 10.1021/ja060729t.
4
Coactivator proteins as determinants of estrogen receptor structure and function: spectroscopic evidence for a novel coactivator-stabilized receptor conformation.共激活蛋白作为雌激素受体结构和功能的决定因素：新型共激活蛋白稳定受体构象的光谱学证据
Mol Endocrinol. 2005 Jun;19(6):1516-28. doi: 10.1210/me.2004-0458. Epub 2005 Jan 20.
5
A proteomic microarray approach for exploring ligand-initiated nuclear hormone receptor pharmacology, receptor selectivity, and heterodimer functionality.一种用于探索配体引发的核激素受体药理学、受体选择性和异二聚体功能的蛋白质组学微阵列方法。
Mol Cell Proteomics. 2005 Mar;4(3):267-77. doi: 10.1074/mcp.M400192-MCP200. Epub 2004 Dec 20.
6
Carbostyril derivatives as antenna molecules for luminescent lanthanide chelates.作为发光镧系螯合物天线分子的咔唑衍生物
Bioconjug Chem. 2004 Sep-Oct;15(5):1088-94. doi: 10.1021/bc049915j.
7
Site-specific fluorescent labeling of estrogen receptors and structure-activity relationships of ligands in terms of receptor dimer stability.雌激素受体的位点特异性荧光标记及配体在受体二聚体稳定性方面的构效关系。
Methods Enzymol. 2003;364:37-53. doi: 10.1016/s0076-6879(03)64003-6.
8
Luminescence resonance energy transfer-based high-throughput screening assay for inhibitors of essential protein-protein interactions in bacterial RNA polymerase.基于发光共振能量转移的高通量筛选分析方法，用于筛选细菌RNA聚合酶中关键蛋白质-蛋白质相互作用的抑制剂。
Appl Environ Microbiol. 2003 Mar;69(3):1492-8. doi: 10.1128/AEM.69.3.1492-1498.2003.
9
Principles and biophysical applications of lanthanide-based probes.基于镧系元素的探针的原理及生物物理应用
Annu Rev Biophys Biomol Struct. 2002;31:275-302. doi: 10.1146/annurev.biophys.31.101101.140927. Epub 2001 Oct 25.
10
Mean DNA bend angle and distribution of DNA bend angles in the CAP-DNA complex in solution.溶液中CAP-DNA复合物的平均DNA弯曲角度及DNA弯曲角度分布。
J Mol Biol. 2001 Sep 21;312(3):453-68. doi: 10.1006/jmbi.2001.4976.
Zotero 插件