Saab-Rincón Gloria, Valderrama Brenda
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Antioxid Redox Signal. 2009 Feb;11(2):167-92. doi: 10.1089/ars.2008.2098.
Redox-active enzymes perform many key biological reactions. The electron transfer process is complex, not only because of its versatility, but also because of the intricate and delicate modulation exerted by the protein scaffold on the redox properties of the catalytic sites. Nowadays, there is a wealth of information available about the catalytic mechanisms of redox-active enzymes and the time is propitious for the development of projects based on the protein engineering of redox-active enzymes. In this review, we aim to provide an updated account of the available methods used for protein engineering, including both genetic and chemical tools, which are usually reviewed separately. Specific applications to redox-active enzymes are mentioned within each technology, with emphasis on those cases where the generation of novel functionality was pursued. Finally, we focus on two emerging fields in the protein engineering of redox-active enzymes: the construction of novel nucleic acid-based catalysts and the remodeling of intra-molecular electron transfer networks. We consider that the future development of these areas will represent fine examples of the concurrence of chemical and genetic tools.
氧化还原活性酶参与许多关键的生物反应。电子转移过程很复杂，这不仅是因为其具有多功能性，还因为蛋白质支架对催化位点的氧化还原特性施加了复杂而精细的调节。如今，关于氧化还原活性酶催化机制的信息丰富，当下正是开展基于氧化还原活性酶蛋白质工程的项目的有利时机。在本综述中，我们旨在提供用于蛋白质工程的现有方法的最新说明，包括遗传工具和化学工具，这两种工具通常是分开进行综述的。每种技术中都提到了氧化还原活性酶的具体应用，重点是那些追求产生新功能的案例。最后，我们关注氧化还原活性酶蛋白质工程中的两个新兴领域：新型核酸基催化剂的构建和分子内电子转移网络的重塑。我们认为这些领域的未来发展将成为化学工具和遗传工具协同作用的典范。
