酶中隧道效应的指导原则。
Guidelines for tunneling in enzymes.
作者信息
Moser Christopher C, Anderson J L Ross, Dutton P Leslie
机构信息
Dept. Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
出版信息
Biochim Biophys Acta. 2010 Sep;1797(9):1573-86. doi: 10.1016/j.bbabio.2010.04.441. Epub 2010 May 10.
Abstract
Here we extend the engineering descriptions of simple, single-electron-tunneling chains common in oxidoreductases to quantify sequential oxidation-reduction rates of two-or-more electron cofactors and substrates. We identify when nicotinamides may be vulnerable to radical mediated oxidation-reduction and merge electron-tunneling expressions with the chemical rate expressions of Eyring. The work provides guidelines for the construction of new artificial oxidoreductases inspired by Nature but adopting independent design and redox engineering.
摘要
在这里，我们扩展了氧化还原酶中常见的简单单电子隧穿链的工程描述，以量化两个或更多电子辅因子和底物的顺序氧化还原速率。我们确定了烟酰胺何时可能易受自由基介导的氧化还原作用影响，并将电子隧穿表达式与艾林的化学速率表达式合并。这项工作为受自然启发但采用独立设计和氧化还原工程的新型人工氧化还原酶的构建提供了指导方针。
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