School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore.
Biotechnol Adv. 2012 May-Jun;30(3):541-9. doi: 10.1016/j.biotechadv.2011.09.008. Epub 2011 Sep 21.
Protein engineering has for decades been a powerful tool in biotechnology for generating vast numbers of useful enzymes for industrial applications. Today, protein engineering has a crucial role in advancing the emerging field of synthetic biology, where metabolic engineering efforts alone are insufficient to maximize the full potential of synthetic biology. This article reviews the advancements in protein engineering techniques for improving biocatalytic properties to optimize engineered pathways in host systems, which are instrumental to achieve high titer production of target molecules. We also discuss the specific means by which protein engineering has improved metabolic engineering efforts and provide our assessment on its potential to continue to advance biology engineering as a whole.
几十年来，蛋白质工程一直是生物技术领域的有力工具，可用于生成大量用于工业应用的有用酶。如今，蛋白质工程在新兴的合成生物学领域发挥着关键作用，仅通过代谢工程的努力不足以充分发挥合成生物学的全部潜力。本文综述了蛋白质工程技术在改善生物催化特性方面的进展，以优化宿主系统中的工程途径，这对于实现目标分子的高产量至关重要。我们还讨论了蛋白质工程改进代谢工程努力的具体方法，并对其继续推动整个生物工程学发展的潜力进行了评估。
