Wang Xin-Rong, Kang Hui-Yuan, Cen Jian, Li Yong-Hui, Wang Li-Li, Yu Li
Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Dec;18(6):1402-4.
This study was purposed to investigate the methylation status of id4 gene promoter in patients with chronic myeloid leukemia (CML) and explore the relationship between methylation of the id4 gene and progress of CML. The methylation status of id4 gene in 48 chronic myeloid leukemia patients and 10 healthy individuals was detected by using methylation-specific polymerase chain reaction (MS-PCR). The results showed that id4 gene was unmethylated in bone marrow samples from both healthy individuals and CML patients in chronic phase (CP). The rate of id4 gene methylation in both CML patients in accelerated phase (AP) and blast crisis (BC) was 66%, and was higher than those of CML patients in CP phase. There was significant difference between them (p < 0.05). In one CML patient who received a serial observations, the status of id4 was unmethylated in CP, but it was methylated in AP and BC phase. It is concluded that the id4 gene in CML patients is unmethylated in CP, while it is methylated in AP or BC. The detection of id4 gene methylation status may be useful for monitoring disease advance in CML and may be used as a marker of disease progression in CML.
本研究旨在探讨慢性髓性白血病（CML）患者中id4基因启动子的甲基化状态，并探索id4基因甲基化与CML进展之间的关系。采用甲基化特异性聚合酶链反应（MS-PCR）检测48例慢性髓性白血病患者和10例健康个体中id4基因的甲基化状态。结果显示，健康个体和慢性期（CP）CML患者的骨髓样本中id4基因均未甲基化。加速期（AP）和急变期（BC）CML患者的id4基因甲基化率均为66%，高于CP期CML患者。两者之间存在显著差异（p<0.05）。在1例接受系列观察的CML患者中，id4基因在CP期未甲基化，但在AP期和BC期甲基化。结论是，CML患者的id4基因在CP期未甲基化，而在AP期或BC期甲基化。检测id4基因甲基化状态可能有助于监测CML疾病进展，并可作为CML疾病进展的标志物。
