Levent Fatma, Greer Jewel M, Snider Malorie, Demmler-Harrison Gail J
Baylor College of Medicine, Department of Pediatrics, Section of Infectious Diseases, Houston, TX, USA.
J Clin Virol. 2009 Feb;44(2):173-5. doi: 10.1016/j.jcv.2008.11.002. Epub 2008 Dec 19.
Adenoviruses are a prominent cause of respiratory, ocular, gastrointestinal, and disseminated diseases in healthy and immunocompromised children. An accurate rapid diagnostic assay may impact clinical decision-making.
Evaluate the performance of a new rapid assay for detection of adenoviruses directly in pediatric clinical specimens.
The rapid assay was performed on adenovirus culture-positive original samples and on an equal number of culture-negative samples matched by patient age and specimen type. Discrepant results were resolved using a polymerase chain reaction (PCR) assay.
200 adenovirus culture-positive and 200 adenovirus culture-negative samples were evaluated from 315 different patients. Overall sensitivity was 55% and specificity was 98.9%. The assay was most sensitive in children 5 years old and younger and most specific in respiratory samples.
The rapid assay was highly specific for detecting adenovirus infections in children. However, since this rapid assay had only moderate to low sensitivity, samples with negative rapid assay results should have additional testing for adenovirus performed by either viral culture or PCR.
腺病毒是健康儿童和免疫功能低下儿童呼吸道、眼部、胃肠道及播散性疾病的主要病因。准确的快速诊断检测可能会影响临床决策。
评估一种直接检测儿科临床标本中腺病毒的新型快速检测方法的性能。
对腺病毒培养阳性的原始样本以及同等数量的按患者年龄和标本类型匹配的培养阴性样本进行快速检测。使用聚合酶链反应（PCR）检测解决不一致的结果。
对来自315名不同患者的200份腺病毒培养阳性样本和200份腺病毒培养阴性样本进行了评估。总体敏感性为55%，特异性为98.9%。该检测方法在5岁及以下儿童中最敏感，在呼吸道样本中最具特异性。
该快速检测方法对检测儿童腺病毒感染具有高度特异性。然而，由于这种快速检测方法的敏感性仅为中度至低度，快速检测结果为阴性的样本应通过病毒培养或PCR对腺病毒进行额外检测。
