约旦儿童腺病毒感染的检测

Detection of adenovirus infection in children in Jordan.

作者信息

Meqdam M M, Nasrallah G, al-Shurman A

机构信息

Department of Applied Biology, Princess Rahma Hospital, Jordan University of Science and Technology, PO Box. 3030, Irbid 22110, Jordan.

出版信息

Ann Trop Paediatr. 2001 Mar;21(1):59-65.

PMID:11284249

Abstract

Between November 1997 and May 1998, 350 nasopharyngeal aspirates (NPA) were obtained from children admitted to the Respiratory Disease Unit at Princess Rahma Hospital, northern Jordan who were clinically diagnosed as suffering from respiratory tract infections. NPA were investigated for the presence of adenovirus using shell vial (SV) culture assay, conventional culture (CC) assay, and direct immunofluorescence assay (DFA). Of the 350 NPA, adenoviruses were detected in 54 (15.4%) by the combined techniques used. SV identified 34 (63%), CC 48 (89%) and DFA 30 (56%). Most virus isolations were in children aged 1-< 5 years old and were associated with pneumonia in 39% and bronchopneumonia in 32%. SV assay showed a sensitivity and specificity of 68.8% and 99.7%, respectively, for detecting adenovirus from NPA. These results emphasize that CC assay is still important for the diagnosis of adenovirus, although SV and DFA are superior diagnostic assays.

摘要

1997年11月至1998年5月期间，从约旦北部拉赫玛公主医院呼吸疾病科收治的临床诊断为患有呼吸道感染的儿童中获取了350份鼻咽抽吸物（NPA）。使用空斑小室（SV）培养测定法、传统培养（CC）测定法和直接免疫荧光测定法（DFA）对NPA进行腺病毒检测。在这350份NPA中，通过所使用的联合技术检测到54份（15.4%）含有腺病毒。SV鉴定出34份（63%），CC鉴定出48份（89%），DFA鉴定出30份（56%）。大多数病毒分离株来自1至<5岁的儿童，其中39%与肺炎相关，32%与支气管肺炎相关。SV测定法检测NPA中腺病毒的灵敏度和特异性分别为68.8%和99.7%。这些结果强调，尽管SV和DFA是更优的诊断测定法，但CC测定法对于腺病毒的诊断仍然很重要。