精子蛋白17的异常表达增强卵巢癌细胞系HO-8910的迁移能力
[Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910].
作者信息
Liu Qun, Li Fang-Qiu, Han Yan-Ling, Wang Li-Kui, Hou Ya-Yi
机构信息
PLA Institute of Clinical Laboratory Medicine, Nanjing University School of Medicine/Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
出版信息
Zhonghua Nan Ke Xue. 2008 Nov;14(11):982-6.
OBJECTIVE
To explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.
METHODS
The recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.
RESULTS
Sp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).
CONCLUSION
The aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
目的
探讨精子蛋白17（Sp17）异常表达对卵巢癌细胞系HO-8910迁移的影响。
方法
用脂质体2000将含Sp17和增强型绿色荧光蛋白基因的重组质粒pEGFP-Sp17转染入人卵巢癌细胞系HO-8910。采用RT-PCR和蛋白质免疫印迹法检测Sp17的表达，用Transwell小室实验检测细胞迁移能力。
结果
转染后Sp17作为与EGFP的融合蛋白表达。转染组和对照组迁移细胞数有显著差异（156.6±14.9/HP对39.3±8.53/HP，P<0.05）。
结论
Sp17的异常表达显著增强卵巢癌细胞的迁移能力。
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