Orsini G, Ruggeri A, Mazzoni A, Nato F, Falconi M, Putignano A, Di Lenarda R, Nanci A, Breschi L
Department of Restorative Dentistry, Institute of Stomatology, Polytechnique University of Marche, Ancona, Italy.
Eur J Histochem. 2008 Oct-Dec;52(4):215-20. doi: 10.4081/1219.
Dentin matrix protein 1 (DMP1) is a non-collagenous matrix protein with a recognized role in the formation of mineralized tissues such as dentin. The aim of this study was to analyze the distribution of DMP1 in human dentin by means of immunofluorescence and high-resolution immunogold labeling. Fully developed, sound human dentin specimens were submitted to fluorescence labeling and post-embedding immunolabeling techniques with a rabbit polyclonal antihuman DMP1 antibody followed by corresponding fluorochrome-conjugated or gold-conjugated secondary antibodies. Both immunofluorescence and immunogold labeling showed an intense labeling associated with the peritubular dentin. In addition, at the ultrastructural level, there was also a moderate and diffuse immunoreaction over intertubular dentin, and a weak labeling within predentin which increased in density towards the mineralization front. This study suggests that in adult human teeth, like in rodents, DMP1 is prevalently concentrated at the level of peritubular dentin and this feature is preserved also in fully developed-teeth. These data are consistent with what has been observed in rodents and suggest that DMP1 plays a role in maintenance of the dentin tubular space.
牙本质基质蛋白1（DMP1）是一种非胶原蛋白基质蛋白，在牙本质等矿化组织的形成中具有公认的作用。本研究的目的是通过免疫荧光和高分辨率免疫金标记分析DMP1在人牙本质中的分布。将完全发育成熟、完好的人牙本质标本用兔抗人DMP1多克隆抗体进行荧光标记和包埋后免疫标记技术处理，随后用相应的荧光染料偶联或金偶联二抗进行处理。免疫荧光和免疫金标记均显示与管周牙本质相关的强烈标记。此外，在超微结构水平上，管间牙本质也有中度弥漫性免疫反应，前期牙本质内有较弱的标记，其密度向矿化前沿增加。本研究表明，在成人牙齿中，与啮齿动物一样，DMP1主要集中在管周牙本质水平，并且这一特征在完全发育的牙齿中也得以保留。这些数据与在啮齿动物中观察到的结果一致，表明DMP1在维持牙本质小管空间中起作用。
