Fisher P J, Malthus B, Walker M C, Corbett G, Spelman R J
Livestock Improvement Corporation Ltd., Hamilton 3240, New Zealand.
J Dairy Sci. 2009 Jan;92(1):369-74. doi: 10.3168/jds.2008-1086.
New platforms utilizing single nucleotide polymorphisms (SNP) offer operational advantages over the conventional microsatellite-based ones, making them a promising alternative for parentage exclusion. Through simulation and empirical data, a 40-SNP panel (where the minor allele frequency was 0.35 on average) was shown to be a comparable or better diagnostic tool than the current 14-microsatellite panel that is used to parentage test New Zealand dairy animals. The 40 SNP alone did not have sufficient power of exclusion to match more than 75% of the progeny to the correct sire and dam. Utilizing mating records and grouping progeny and dams by birth and calving dates, respectively, decreased the number of sire-dam combinations that each progeny was tested against and dramatically increased the utility of the SNP. These results highlight the importance of combining genotypes with on-farm data to maximize the ability to assign parentage in the New Zealand dairy herd.
利用单核苷酸多态性（SNP）的新平台比传统的基于微卫星的平台具有操作优势，使其成为亲权排除的一个有前途的替代方案。通过模拟和实证数据表明，一个由40个SNP组成的面板（平均次要等位基因频率为0.35）是一种与目前用于新西兰奶牛亲子鉴定的14个微卫星面板相当或更好的诊断工具。仅这40个SNP没有足够的排除能力来将超过75%的后代与正确的父本和母本匹配。分别利用配种记录以及按出生和产犊日期对后代和母本进行分组，减少了每个后代所检测的父本-母本组合数量，并显著提高了SNP的效用。这些结果突出了将基因型与农场数据相结合以最大化在新西兰奶牛群中确定亲权能力的重要性。
