Wu Josephine, Zhang Wandi, Xie Boxun, Wu Maoxin, Tong Xiaodi, Kalpoe Jayant, Zhang David
Department of Pathology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1122, New York, New York 10029, USA.
J Clin Microbiol. 2009 Mar;47(3):807-10. doi: 10.1128/JCM.01324-08. Epub 2008 Dec 24.
Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.
由于目前的微生物学方法不适用于检测福尔马林固定、石蜡包埋组织样本中的产气荚膜梭菌,我们开发了一种聚合酶链反应(PCR)检测方法,以检测产气荚膜梭菌的毒素编码基因和16S核糖体RNA(rRNA)基因。我们成功地在两例尸检病例的组织切片中检测到产气荚膜梭菌并进行了基因分型。