Mwaki Daniel M, Kidambasi Kevin O, Kinyua Johnson, Ogila Kenneth, Kigen Collins, Getange Dennis, Villinger Jandouwe, Masiga Daniel K, Carrington Mark, Bargul Joel L
Animal Health Department/Molecular Biology and Bioinformatics Unit, International Centre of Insect Physiology and Ecology (icipe), Nairobi, P.O. BOX 30772-00100, Kenya.
Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi, P.O. BOX 62000-00200, Kenya.
Open Res Afr. 2022 Jun 6;5:23. doi: 10.12688/openresafrica.13404.1. eCollection 2022.
Livestock are key sources of livelihood among pastoral communities. Livestock productivity is chiefly constrained by pests and diseases. Due to inadequate disease surveillance in northern Kenya, little is known about pathogens circulating within livestock and the role of livestock-associated biting keds (genus ) in disease transmission. We aimed to identify the prevalence of selected hemopathogens in livestock and their associated blood-feeding keds. We randomly collected 389 blood samples from goats (245), sheep (108), and donkeys (36), as well as 235 keds from both goats and sheep (116), donkeys (11), and dogs (108) in Laisamis, Marsabit County, northern Kenya. We screened all samples for selected hemopathogens by high-resolution melting (HRM) analysis and sequencing of PCR products amplified using primers specific to the genera: and In goats, we detected (84.5%), a novel sp. (11.8%), (7.3%), (66.1%), and (0.8%). We also detected (93.5%), (22.2%), and (38.9%) in sheep. In donkeys, we detected ' Anaplasma camelii' (11.1%), (22.2%), (25%), and (13.9%). In addition, keds carried the following pathogens; goat/sheep keds - (29.3%) (0.86%), (0.86%), and (51.7%); donkey keds - (18.2%) and (63.6%); and dog keds - (15.7%), (0.9%), (0.9%) (76%), (46.3%), (76%), and (5.6%). We found that livestock and their associated ectoparasitic biting keds carry a number of infectious hemopathogens, including the zoonotic . Dog keds harbored the most pathogens, suggesting dogs, which closely interact with livestock and humans, as key reservoirs of diseases in Laisamis. These findings can guide policy makers in disease control.
牲畜是牧区生计的关键来源。牲畜生产力主要受到病虫害的制约。由于肯尼亚北部疾病监测不足，人们对牲畜体内传播的病原体以及与牲畜相关的吸血蜱（属）在疾病传播中的作用知之甚少。我们旨在确定牲畜及其相关吸血蜱中选定血液病原体的流行情况。我们在肯尼亚北部马萨比特县莱萨米斯随机采集了389份血液样本，其中山羊245份、绵羊108份、驴36份，以及来自山羊和绵羊的235只蜱（116只）、驴11只和狗108只。我们通过高分辨率熔解（HRM）分析和使用针对以下属的特异性引物扩增的PCR产物测序，对所有样本进行选定血液病原体的筛查：和。在山羊中，我们检测到（84.5%）、一种新型（11.8%）、（7.3%）、（66.1%）和（0.
