Yoo H S, Lee S U, Park K Y, Park Y H
Department of Bacteriology and Immunology, National Veterinary Research Institute, Kyungg, Republic of Korea.
J Clin Microbiol. 1997 Jan;35(1):228-32. doi: 10.1128/jcm.35.1.228-232.1997.
Clostridium perfringens has been classified into five toxigenic types (A through E) on the basis of its capability to produce major lethal toxins (alpha, beta, epsilon, and iota toxins). Seroneutralization with mice or guinea pigs has been used to type each toxin, but this conventional method has some disadvantages. Therefore, we used a molecular biological technique to type the bacterium in the present study. A multiplex PCR was developed for this purpose. This method has several advantages in comparison with seroneutralization with mice or guinea pigs. By this method, we also investigated the most prevalent type(s) of the organism in Korean calves, piglets, and chickens showing clinical symptoms such as diarrhea, enterotoxemia, and necrotic enteritis. Only type A was isolated from calves and chickens, while type C (2 of 14 isolates), in addition to type A, was isolated from piglets. These results suggested that seroneutralization could be replaced by our new method and that type A of C. perfringens is the most prevalent type in livestock in Korea.
产气荚膜梭菌已根据其产生主要致死毒素（α、β、ε和ι毒素）的能力被分为五种产毒类型（A至E）。使用小鼠或豚鼠进行血清中和试验来对每种毒素进行分型，但这种传统方法存在一些缺点。因此，在本研究中我们使用分子生物学技术对该细菌进行分型。为此开发了一种多重聚合酶链反应（multiplex PCR）。与使用小鼠或豚鼠进行血清中和试验相比，该方法具有几个优点。通过这种方法，我们还调查了韩国出现腹泻、肠毒血症和坏死性肠炎等临床症状的犊牛、仔猪和鸡中该菌最常见的类型。仅从犊牛和鸡中分离出A型，而从仔猪中除分离出A型外，还分离出C型（14株分离株中有2株）。这些结果表明，血清中和试验可被我们的新方法取代，并且产气荚膜梭菌A型是韩国家畜中最常见的类型。
