Kloth Katrin, Niessner Reinhard, Seidel Michael
Institute of Hydrochemistry and Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, D-81377 München, Germany.
Biosens Bioelectron. 2009 Mar 15;24(7):2106-12. doi: 10.1016/j.bios.2008.11.005. Epub 2008 Nov 18.
A novel automated chemiluminescence (CL) read-out system for analytical flow-through microarrays based on multiplexed immunoassays has been developed. The microarray chip reader (MCR 3) is designed as a stand-alone platform, with the goal to quantify multiple analytes in complex matrices of food and liquid samples for field analysis or for routine analytical laboratories. The CL microarray platform is a self-contained system for the fully automated multiplexed immunoanalysis: the microarray chip, the fluidic system and the software module enable automated calibration and determination of analyte concentrations during a whole working day. The detection of antibiotics in milk was demonstrated to validate this device. There are few quantitative multi-residue detection methods for routine analysis although the EU has defined maximum residue limits (MRLs) for a number of antibacterial reagents. Therefore, an automated multianalyte detection instrument is needed quantifying simultaneously antibiotics within some minutes. Also regeneration is required to avoid replacing the assay surface. The MCR 3 uses a microarray chip, which consists of two channels for parallel measurement and regeneration. The microarray chip is designed for parallel analysis of up to 13 different antibiotics in milk applying an indirect competitive microarray immunoassay (MIA). Microspotted antibiotics are directly coupled to epoxylated PEG surfaces. As an initial example, penicillin G is quantified in milk on the MCR 3. The penicillin G surface is regenerable for 47 measurement cycles per channel. A limit of detection (LOD) of 1.1 microg/L is achieved by an assay time of 6 min.
已开发出一种基于多重免疫分析的用于分析流通式微阵列的新型自动化化学发光（CL）读出系统。微阵列芯片读数器（MCR 3）被设计成一个独立平台，目标是对食品和液体样品的复杂基质中的多种分析物进行定量，用于现场分析或常规分析实验室。CL微阵列平台是一个用于全自动多重免疫分析的独立系统：微阵列芯片、流体系统和软件模块能够在整个工作日实现自动校准和分析物浓度测定。通过检测牛奶中的抗生素来验证该设备。尽管欧盟已为多种抗菌试剂定义了最大残留限量（MRL），但用于常规分析的定量多残留检测方法却很少。因此，需要一种能在几分钟内同时定量抗生素的自动化多分析物检测仪器。同时还需要再生以避免更换检测表面。MCR 3使用一种微阵列芯片，该芯片由两个用于平行测量和再生的通道组成。该微阵列芯片设计用于应用间接竞争微阵列免疫分析（MIA）对牛奶中多达13种不同抗生素进行平行分析。微点样的抗生素直接偶联到环氧聚乙二醇表面。作为一个初始示例，在MCR 3上对牛奶中的青霉素G进行定量。青霉素G表面每个通道可进行47个测量循环的再生。通过6分钟的分析时间实现了1.1微克/升的检测限。
