Du Hongwu, Yang Weiping, Xing Wanli, Su Yao, Cheng Jing
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
Biomed Microdevices. 2005 Jun;7(2):143-6. doi: 10.1007/s10544-005-1594-z.
A protein microarray system for detection and quantification of nine prohibited drugs in serum is described. Chemically modified slides were chosen as the microarray substrates because of their suitable for drug-BSA printing. The developed protein microarray was able to preserve the biological function of the haptens, when immobilized on the microarray surface and demonstrated binding with their corresponding antibodies. The microarray could also be used for quantitative analysis when mouse IgG was chosen as an internal control for data processing. There was no qualitative difference between the results obtained using the protein microarray and ELISA. The protein microarray technology should be applicable to performing, simultaneously, large scale screening tests for many different analytes in serum.
本文描述了一种用于检测和定量血清中九种违禁药物的蛋白质微阵列系统。由于化学修饰的玻片适合药物-牛血清白蛋白（BSA）打印，因此被选作微阵列基质。所开发的蛋白质微阵列在固定于微阵列表面时能够保留半抗原的生物学功能，并证明能与其相应抗体结合。当选择小鼠IgG作为数据处理的内参时，该微阵列还可用于定量分析。使用蛋白质微阵列和酶联免疫吸附测定（ELISA）获得的结果在定性上没有差异。蛋白质微阵列技术应适用于同时对血清中多种不同分析物进行大规模筛查测试。
