Li Nan, Shen Minjie, Xu Youchun
State Key Laboratory of Membrane Biology, Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China.
Micromachines (Basel). 2021 Mar 24;12(4):347. doi: 10.3390/mi12040347.
Protein biomarkers are indicators of many diseases and are commonly used for disease diagnosis and prognosis prediction in the clinic. The urgent need for point-of-care (POC) detection of protein biomarkers has promoted the development of automated and fully sealed immunoassay platforms. In this study, a portable microfluidic system was established for the POC detection of multiple protein biomarkers by combining a protein microarray for a multiplex immunoassay and a microfluidic cassette for reagent storage and liquid manipulation. The entire procedure for the immunoassay was automatically conducted, which included the antibody-antigen reaction, washing and detection. Alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA125) were simultaneously detected in this system within 40 min with limits of detection of 0.303 ng/mL, 1.870 ng/mL, and 18.617 U/mL, respectively. Five clinical samples were collected and tested, and the results show good correlations compared to those measured by the commercial instrument in the hospital. The immunoassay cassette system can function as a versatile platform for the rapid and sensitive multiplexed detection of biomarkers; therefore, it has great potential for POC diagnostics.
蛋白质生物标志物是多种疾病的指标，在临床上常用于疾病诊断和预后预测。对蛋白质生物标志物进行即时检测（POC）的迫切需求推动了自动化和全封闭免疫分析平台的发展。在本研究中，通过结合用于多重免疫分析的蛋白质微阵列和用于试剂储存及液体处理的微流控盒，建立了一种用于即时检测多种蛋白质生物标志物的便携式微流控系统。免疫分析的整个过程自动进行，包括抗体 - 抗原反应、洗涤和检测。在该系统中，甲胎蛋白（AFP）、癌胚抗原（CEA）和癌抗原125（CA125）在40分钟内同时被检测到，检测限分别为0.303 ng/mL、1.870 ng/mL和18.617 U/mL。收集并检测了5份临床样本，结果与医院商用仪器测量的结果相比显示出良好的相关性。免疫分析盒系统可作为一种用于生物标志物快速灵敏多重检测的通用平台；因此，它在即时诊断方面具有巨大潜力。
