Warnke Molly M, Breitbach Zachary S, Dodbiba Edra, Wanigasekara Eranda, Zhang Xiaotong, Sharma Pritesh, Armstrong Daniel W
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, Texas 76019, USA.
J Am Soc Mass Spectrom. 2009 Mar;20(3):529-38. doi: 10.1016/j.jasms.2008.11.015. Epub 2008 Nov 27.
A general and sensitive method for detecting divalent anions by ESI-MS and LC/ESI-MS as positive ions has been developed. The anions are paired with tricationic reagents to form positively charged complexes. In this study, four tricationic reagents, 2 trigonal and 2 linear, were used to study a wide variety of anions, such as disulfonates, dicarboxylates, and inorganic anions. The limits of detection for many of the anions studied were often improved by tandem mass spectrometry. Tricationic pairing agents can also be used with chromatography to enhance the detection of anions. The tricationic reagents were also used to detect monovalent anions by monitoring the doubly charged positive complex. The limits of detection for some of the divalent anions by this method are substantially lower than by other current analytical techniques.
已开发出一种通过电喷雾电离质谱法（ESI-MS）和液相色谱/电喷雾电离质谱法（LC/ESI-MS）以正离子形式检测二价阴离子的通用且灵敏的方法。阴离子与三价阳离子试剂配对形成带正电荷的络合物。在本研究中，使用了四种三价阳离子试剂（2种三角型和2种线型）来研究多种阴离子，如二磺酸盐、二羧酸盐和无机阴离子。串联质谱法常常能提高许多所研究阴离子的检测限。三价阳离子配对剂也可与色谱法联用，以增强阴离子的检测效果。这些三价阳离子试剂还通过监测双电荷正络合物来检测单价阴离子。用此方法检测某些二价阴离子的检测限明显低于其他现有分析技术。
