Barreteau Hélène, Magnet Sophie, El Ghachi Meriem, Touzé Thierry, Arthur Michel, Mengin-Lecreulx Dominique, Blanot Didier
Université Paris-Sud, UMR 8619, Institut de Biochimie et Biophysique Moléculaire et Cellulaire, 91405 Orsay Cedex, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):213-20. doi: 10.1016/j.jchromb.2008.12.010. Epub 2008 Dec 11.
Undecaprenyl phosphate is the essential lipid involved in the transport of hydrophilic motifs across the bacterial membranes during the synthesis of cell wall polymers such as peptidoglycan. A HPLC procedure was developed for the quantification of undecaprenyl phosphate and its two derivatives, undecaprenyl pyrophosphate and undecaprenol. During the exponential growth phase, the pools of undecaprenyl phosphate and undecaprenyl pyrophosphate were ca. 75 and 270 nmol/g of cell dry weight, respectively, in Escherichia coli, and ca. 50 and 150 nmol/g, respectively, in Staphylococcus aureus. Undecaprenol was detected in S. aureus (70 nmol/g), but not in E. coli (<1 nmol/g).
十一异戊二烯磷酸酯是在细胞壁聚合物（如肽聚糖）合成过程中，参与亲水性基序跨细菌膜运输的必需脂质。开发了一种高效液相色谱法（HPLC）来定量测定十一异戊二烯磷酸酯及其两种衍生物，即十一异戊二烯焦磷酸酯和十一异戊二烯醇。在指数生长期，大肠杆菌中十一异戊二烯磷酸酯和十一异戊二烯焦磷酸酯的含量分别约为75和270 nmol/g细胞干重，金黄色葡萄球菌中分别约为50和150 nmol/g。在金黄色葡萄球菌中检测到了十一异戊二烯醇（70 nmol/g），但在大肠杆菌中未检测到（<1 nmol/g）。
