Yokoi H, Watanabe T, Fujii Y, Mukai T, Toba T, Adachi S
Hiratsuka Research Laboratory, Sumitomo Heavy Industries, Ltd., Kanagawa, Japan.
Int J Food Microbiol. 1991 Aug;13(4):257-64. doi: 10.1016/0168-1605(91)90083-2.
A capsular polysaccharide-producing strain KPB-167B isolated from kefir grains was identified as a homofermentative Lactobacillus. The carbohydrate fermentation pattern and DNA base composition of the strain were different from those of other capsular Lactobacillus species previously isolated from kefir grains. The polysaccharide produced by Lactobacillus sp. KPB-167B was found similar to kefiran by 13C-NMR and methylation analysis. Lactobacillus sp. KPB-167B could grow and produce capsular polysaccharide in MRSL medium with better yield than L. kefiranofaciens, which suggested that it is suitable for kefiran production.
从开菲尔粒中分离出的一株产荚膜多糖的菌株KPB-167B被鉴定为同型发酵乳杆菌。该菌株的碳水化合物发酵模式和DNA碱基组成与先前从开菲尔粒中分离出的其他荚膜乳杆菌种类不同。通过13C-NMR和甲基化分析发现，乳杆菌属KPB-167B产生的多糖与开菲尔多糖相似。乳杆菌属KPB-167B能够在MRSL培养基中生长并产生荚膜多糖，其产量高于开菲尔糖乳杆菌，这表明它适合用于生产开菲尔多糖。
