Área Bioquímica y Control de Alimentos, Dpto. Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, La Plata, Buenos Aires, Argentina; Dpto. de Química Aplicada, Facultad de Ciencias Químicas, Universidad del País Vasco (UPV/EHU), Paseo Manuel de Lardizabal 3, San Sebastián, País Vasco, Spain.
Dpto. de Química Aplicada, Facultad de Ciencias Químicas, Universidad del País Vasco (UPV/EHU), Paseo Manuel de Lardizabal 3, San Sebastián, País Vasco, Spain.
Carbohydr Polym. 2017 Aug 15;170:52-59. doi: 10.1016/j.carbpol.2017.04.053. Epub 2017 Apr 23.
Lactobacillus plantarum CIDCA 8327 is an exopolysaccharide (EPS)-producer strain isolated from kefir with promising properties for the development of functional foods. The aim of the present study was to characterize the structure of the EPS synthesized by this strain grown in skim milk or semidefined medium (SDM). Additionally, genes involved in EPS synthesis were detected by PCR. L. plantarum produces an EPS with a molecular weight of 10Da in both media. When grown in SDM produce an heteropolysaccharide composed mainly of glucose, glucosamine and rhamnose meanwhile the EPS produced in milk was composed exclusively of glucose indicating the influence of the sugar source. FTIR spectra of this EPS showed signals attributable to an α-glucan. Both by H NMR and methylation analysis it was possible to determine that this polysaccharide is a branched α-(1→4)-d-glucan composed of 80% linear α-(1→4)-d-glucopyranosyl units and 19% (1→4)-d-glucopyranosyl units substituted at O-3 by single α-d-glucopyranosil residues.
植物乳杆菌 CIDCA 8327 是从开菲尔中分离出来的一种胞外多糖 (EPS) 产生菌株，具有开发功能性食品的巨大潜力。本研究旨在对该菌株在脱脂乳或半定义培养基 (SDM) 中生长合成的 EPS 结构进行表征。此外，通过 PCR 检测了参与 EPS 合成的基因。植物乳杆菌在两种培养基中均产生分子量为 10Da 的 EPS。在 SDM 中生长时，会产生一种由葡萄糖、葡糖胺和鼠李糖组成的杂多糖，而在牛奶中产生的 EPS 仅由葡萄糖组成，表明糖源的影响。该 EPS 的 FTIR 光谱显示出归因于α-葡聚糖的信号。通过 1H NMR 和甲基化分析，可以确定这种多糖是一种支链α-(1→4)-d-葡聚糖，由 80%的线性α-(1→4)-d-葡萄糖吡喃基单元和 19%的 (1→4)-d-葡萄糖吡喃基单元组成，这些单元在 O-3 处被单个α-d-葡萄糖吡喃基残基取代。
