Lamm C G, Broaddus C C, Holyoak G R
Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater, OK 74076-7001, USA.
Vet Pathol. 2009 Jan;46(1):54-8. doi: 10.1354/vp.46-1-54.
Bovine viral diarrhea virus (BVDV) infection in goats can result in severe reproductive losses, with abortion rates reaching 80%. Infection with BVDV in aborted goat fetuses and stillborn kids can result in placentitis, encephalitis, myocarditis, and thymic depletion. This study investigates the distribution of viral antigen within the organ systems of aborted goat fetuses, stillborn kids, and nonviable kids infected with BVDV at various stages of gestation using immunohistochemistry (IHC). Virus antigen was detected within the placenta (8/13), thymus (4/9), heart (4/11), and brain (4/15) of affected goats. Uncommonly, BVDV antigen was detected within the skin (1/14), liver (1/13), kidney (1/12), lung (1/11), and trachea (1/3). BVDV antigen was not detected within the spleen (0/9), nasal turbinate (0/2), or thyroid (0/3). The results of this study indicate that placenta, heart, thymus, and brain are the most reliable tissues for BVDV antigen detection using IHC in aborted goat fetuses.
山羊感染牛病毒性腹泻病毒（BVDV）可导致严重的繁殖损失，流产率高达80%。流产山羊胎儿和死产羔羊感染BVDV可导致胎盘炎、脑炎、心肌炎和胸腺萎缩。本研究采用免疫组织化学（IHC）方法，调查了在不同妊娠阶段感染BVDV的流产山羊胎儿、死产羔羊和夭折羔羊的器官系统内病毒抗原的分布情况。在受影响山羊的胎盘（8/13）、胸腺（4/9）、心脏（4/11）和大脑（4/15）中检测到病毒抗原。罕见的是，在皮肤（1/14）、肝脏（1/13）、肾脏（1/12）、肺（1/11）和气管（1/3）中检测到BVDV抗原。在脾脏（0/9）、鼻甲骨（0/2）或甲状腺（0/3）中未检测到BVDV抗原。本研究结果表明，对于使用免疫组织化学方法在流产山羊胎儿中检测BVDV抗原而言，胎盘、心脏、胸腺和大脑是最可靠的组织。
