伊朗牛、绵羊、山羊、水牛和骆驼流产胎儿中牛病毒性腹泻病毒的抗原捕获 ELISA 和 RT-PCR 评估的流行率研究。
Prevalence study of Bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in Bovine, Ovine, Caprine, Buffalo and Camel aborted fetuses in Iran.
机构信息
Young Researchers Club, Faculty of Veterinary Medcine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
出版信息
AMB Express. 2011 Oct 21;1(1):32. doi: 10.1186/2191-0855-1-32.
Bovine viral diarrhea virus is a pestivirus in the family Flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. In this study, for more sensitive, specific detection and determined the prevalence of virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses the antigen capture ELISA and RT-PCR were recommended. From the total of 2173 aborted fetuses, 347 (15.96%) and 402 (18.49%) were positive for presence of Bovine viral diarrhea virus by antigen capture ELISA and RT-PCR respectively. Statistical analysis of data showed significant differences between ELISA and RT-PCR for detection of virus in aborted fetuses.These results indicate a high presence of this pathogen in Iran and that RT- PCR is considerably faster and more accurate than ELISA for identification of Bovine viral diarrhea virus.To our knowledge the Camels and Bovine are the most resistant and sensitive to Bovine viral diarrhea's abortions respectively and the prevalence of virus in Caprine is more than Ovine aborted fetuses. This study is the first prevalence report of Bovine viral diarrhea virus in aborted Bovine, Ovine, Caprine, Buffalo and Camel fetuses by evaluation of ELISA and RT-PCR in Iran.
牛病毒性腹泻病毒是黄病毒科的一种瘟病毒，可导致牲畜流产和死产，其传统诊断基于细胞培养和病毒中和试验。在这项研究中，为了更灵敏、更特异的检测并确定流产牛、羊、山羊、水牛和骆驼胎儿中病毒的流行情况，推荐使用抗原捕获 ELISA 和 RT-PCR。从总共 2173 个流产胎儿中，抗原捕获 ELISA 和 RT-PCR 分别检测到 347（15.96%）和 402（18.49%）的牛病毒性腹泻病毒呈阳性。数据的统计分析表明，ELISA 和 RT-PCR 检测流产胎儿中病毒的存在有显著差异。这些结果表明该病原体在伊朗的存在率较高，并且 RT-PCR 比 ELISA 更快速、更准确地鉴定牛病毒性腹泻病毒。据我们所知，骆驼对牛病毒性腹泻的流产最具有抵抗力和敏感性，而山羊流产胎儿中病毒的流行率高于绵羊。本研究是在伊朗首次通过评估 ELISA 和 RT-PCR 对流产牛、羊、山羊、水牛和骆驼胎儿中牛病毒性腹泻病毒进行的流行情况报告。
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