Ma Hong-xia, You Zhao-ling, Wang Ruo-guang
The First Affiliated Hospital of Guangzhou Medicine college, Guangzhou 510405, China.
Zhong Yao Cai. 2008 Aug;31(8):1201-4.
To study the effect of TFCC on expression of Th type-1/Th type-2 cytokines and PR in rats abortion model, and observe the changes of serum P and PRL.
Early abortion rat model was established by Bromocriptine at 6-8 gestation day, the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control. Progesterone and prolactin levels in the serum were measured by radioimmunoassay, the protein expression of PR was inspected by immunohistochemical, RT-PCR was applied to determine the expression of Thl/Th2 mRNA on deciduas.
In the deciduas of model group, IL-4 and IL-10 cytokine contens were clearly lower than that of normal pregnancy group. The difference was notable(P < 0.05), In the deciduas of model group, IFN-gamma and TNF-alpha cytokine contents were clearly higher than that of normal pregnancy group. The difference was notable (P < 0.05), TFCC could up-regulate the level of IL-4 and IL-10, and down-regulate the level of TNF-alpha and IFN-gamma, which could promote P secretion and up regulate PR mRNA expression on deciduas.
TFCC can regulate endocrinologycal and immunological network equilibration on maternal-fetal face, finally achieve prevention abortion.
研究滋养细胞穿通蛋白（TFCC）对大鼠流产模型中Th1/Th2型细胞因子表达及孕激素受体（PR）的影响，并观察血清孕酮（P）和催乳素（PRL）的变化。
于妊娠6 - 8天用溴隐亭建立早期流产大鼠模型，模型大鼠分别给予高低剂量TFCC治疗，以孕酮作为阳性对照。采用放射免疫法测定血清中孕酮和催乳素水平，免疫组织化学法检测PR蛋白表达，逆转录聚合酶链反应（RT-PCR）法测定蜕膜组织中Th1/Th2 mRNA表达。
模型组蜕膜中白细胞介素-4（IL-4）和白细胞介素-10（IL-10）细胞因子含量明显低于正常妊娠组，差异有统计学意义（P < 0.05）；模型组蜕膜中γ-干扰素（IFN-γ）和肿瘤坏死因子-α（TNF-α）细胞因子含量明显高于正常妊娠组，差异有统计学意义（P < 0.05）。TFCC可上调IL-4和IL-10水平，下调TNF-α和IFN-γ水平，促进P分泌并上调蜕膜组织中PR mRNA表达。
TFCC可调节母胎界面的内分泌和免疫网络平衡，最终达到预防流产的目的。
