Hudić Igor, Fatusić Zlatan
Clinic for Gynecology and Obstetrics, University Clinical Center, Tuzla, Bosnia and Herzegovina.
J Perinat Med. 2009;37(4):338-42. doi: 10.1515/JPM.2009.061.
The aim of this prospective study was to compare serum and urine concentrations of progesterone-induced blocking factor (PIBF) and serum concentrations of anti-inflammatory (IL10) and pro-inflammatory (IL6, TNFalpha, IFNgamma) cytokines of women with threatened spontaneous abortion with normal pregnancy and to evaluate the impact of PIBF on outcome of pregnancy.
A sample of 30 women with threatened spontaneous abortion (study group) and 20 healthy pregnant women (control group) between 6(th) and 24(th) gestational weeks was studied. Serum and urine PIBF, IL10 and IL6, TNFalpha, IFNgamma cytokine concentrations were measured by enzyme-linked immunosorbent assay (ELISA).
Five (16.7%) pregnancies in the study group ended missed abortion vs. none in the control group (P<0.05). Five (20%) threatened aborters delivered between 24(th) and 37(th) weeks of gestation, whereas two (10%) preterm deliveries occurred in the controls (P>0.05). PIBF concentrations in urine (19.5+/-12.9 ng/mL) and serum (214.4+/-120.6 of patients with threatened abortion were significantly lower than in healthy pregnant women (45.3+/-33.7 ng/mL and 357.3+/-159.9 ng/mL, respectively). Women with threatened abortion had significantly lower serum levels of anti-inflammatory cytokine, but levels of proinflammatory cytokines were higher in this group compared with healthy controls.
Determination of progesteron-induced blocking factor level in body fluids in early pregnancy might be used for the diagnosis and prognosis of threatened abortion.
本前瞻性研究旨在比较自然流产高危孕妇与正常孕妇血清和尿液中孕酮诱导封闭因子（PIBF）的浓度，以及抗炎细胞因子（IL10）和促炎细胞因子（IL6、TNFα、IFNγ）的血清浓度，并评估PIBF对妊娠结局的影响。
选取30例妊娠6至24周的自然流产高危孕妇（研究组）和20例健康孕妇（对照组）作为研究对象。采用酶联免疫吸附测定法（ELISA）检测血清和尿液中的PIBF、IL10以及IL6、TNFα、IFNγ细胞因子浓度。
研究组中有5例（16.7%）妊娠以稽留流产告终，而对照组无稽留流产发生（P<0.05）。5例（20%）自然流产高危孕妇在妊娠24至37周分娩，而对照组有2例（10%）发生早产（P>0.05）。自然流产高危孕妇尿液中PIBF浓度（19.5±12.9 ng/mL）和血清中PIBF浓度（214.4±120.6 ng/mL）显著低于健康孕妇（分别为45.3±33.7 ng/mL和357.3±159.9 ng/mL）。自然流产高危孕妇血清中抗炎细胞因子水平显著较低，但该组促炎细胞因子水平高于健康对照组。
测定孕早期体液中孕酮诱导封闭因子水平可用于自然流产高危的诊断和预后评估。
