脯氨酸转运蛋白P和渗透压应激蛋白U在小肠结肠炎耶尔森菌于寒冷和渗透应激条件下摄取甜菜碱中的作用。
Role of proP and proU in betaine uptake by Yersinia enterocolitica under cold and osmotic stress conditions.
作者信息
Annamalai Thirunavukkarasu, Venkitanarayanan Kumar
机构信息
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA.
出版信息
Appl Environ Microbiol. 2009 Mar;75(6):1471-7. doi: 10.1128/AEM.01644-08. Epub 2008 Dec 29.
Abstract
Yersinia enterocolitica is a food-borne pathogen with the ability to grow at cold temperatures and tolerate high osmolarity. The bacterium tolerates osmotic stress by intracellular accumulation of osmolytes, such as betaine. The proP gene and proU operon of Y. enterocolitica were sequenced, and single (ProP(-) ProU(+) and ProP(+) ProU(-)) and double (ProP(-) ProU(-)) mutants were generated. Upon exposure to osmotic or chill stress, the single and double mutants demonstrated a reduction in betaine uptake compared to that in the wild type, suggesting that proP and proU play a role in betaine uptake during osmotic and chill stress responses of Y. enterocolitica.
摘要
小肠结肠炎耶尔森菌是一种食源性病原体，能够在低温下生长并耐受高渗透压。该细菌通过细胞内积累渗透保护剂（如甜菜碱）来耐受渗透胁迫。对小肠结肠炎耶尔森菌的proP基因和proU操纵子进行了测序，并构建了单突变体（ProP(-) ProU(+)和ProP(+) ProU(-)）和双突变体（ProP(-) ProU(-)）。在暴露于渗透或冷胁迫时，与野生型相比，单突变体和双突变体的甜菜碱摄取量均降低，这表明proP和proU在小肠结肠炎耶尔森菌的渗透和冷胁迫应答过程中对甜菜碱摄取起作用。
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