氢化酶在鼠伤寒沙门氏菌厌氧产生氢气的循环利用中的作用。
Role of the Hya hydrogenase in recycling of anaerobically produced H2 in Salmonella enterica serovar Typhimurium.
作者信息
Zbell Andrea L, Maier Robert J
机构信息
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
出版信息
Appl Environ Microbiol. 2009 Mar;75(5):1456-9. doi: 10.1128/AEM.02064-08. Epub 2008 Dec 29.
Abstract
Double and triple uptake-type hydrogenase mutants were used to determine which hydrogenase recycles fermentatively produced hydrogen. The Deltahyb Deltahya and Deltahyd Deltahya double mutants evolved H(2) at rates similar to that of the triple mutant strain, so Hya alone oxidizes the bulk of H(2) produced during fermentation. When only Hya was present, no hydrogen production was observed in nutrient-limited medium. H(2) uptake assays showed that Hya can oxidize both exogenously added H(2) and formate hydrogen lyase-evolved H(2) anaerobically. Even after anaerobic growth, all three uptake-type hydrogenases could function in the presence of oxygen, including using O(2) as a terminal acceptor.
摘要
利用双摄取型和三摄取型氢化酶突变体来确定哪种氢化酶可循环利用发酵产生的氢气。ΔhybΔhya和ΔhydΔhya双突变体产生氢气的速率与三突变体菌株相似，因此仅Hya就能氧化发酵过程中产生的大部分氢气。当仅存在Hya时，在营养受限的培养基中未观察到氢气产生。氢气摄取试验表明，Hya能够厌氧氧化外源添加的氢气以及甲酸氢裂解酶产生的氢气。即使在厌氧生长后，所有三种摄取型氢化酶在有氧条件下仍可发挥作用，包括将氧气用作末端受体。
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