Osisanya J O
Department of Pathology and Medical Microbiology, College of Health Sciences, Usmanu Danfodio University, Sokoto, Nigeria.
West Afr J Med. 1991 Apr-Jun;10(2):181-6.
The present study has looked into relative agreement between Clinical diagnosis, Indirect fluorescent Antibody Test and Iso-enzyme grouping in 35 patients with E histolytica infection. Thirty Caucasians, who had never travelled to Tropical Countries were chosen as negative control subjects. There was no remarkable difference in the mean amoebic antibody levels observed with the control subjects and patients harbouring amoebic zymodeme I or III or IV. Whereas, comparative analysis revealed a significant difference (P less than 0.01) between the mean reciprocal antibody titres observed with patients harbouring Isoenzyme group II amoebae and control subjects. The hypothesis that invasive amoebiasis and infection with E. histolytica zymodeme II were positively related was supported IFAT was found more sensitive than isoenzyme grouping in the detection of invasive amoebiasis, but the latter method appeared more reliable in differentiating dysenteric from asymptomatic intestinal amoebiasis.
本研究调查了35例溶组织内阿米巴感染患者的临床诊断、间接荧光抗体试验和同工酶分组之间的相对一致性。选取30名从未去过热带国家的白种人作为阴性对照受试者。在对照受试者与携带阿米巴酶型I、III或IV的患者中观察到的平均阿米巴抗体水平没有显著差异。然而，比较分析显示，携带同工酶II组阿米巴的患者与对照受试者的平均抗体滴度倒数之间存在显著差异（P小于0.01）。侵袭性阿米巴病与溶组织内阿米巴酶型II感染呈正相关这一假设得到了支持。在检测侵袭性阿米巴病方面，间接荧光抗体试验比同工酶分组更敏感，但后一种方法在区分痢疾性与无症状肠道阿米巴病方面似乎更可靠。
