Osisanya J O
J Trop Med Hyg. 1985 Aug;88(4):235-41.
Entamoeba histolytica isolates characterized into four zymodemes were used as antigens in the IFAT; with 17 sera obtained from patients harbouring different amoebic isoenzyme groups. The method of cultivation used was found to influence the ease of reading the IFAT. Reactivity of serum samples, obtained from the selected patients (i.e. with varied forms of amoebiasis), against homologous and heterologous antigens did not reveal any antigenic differences among amoebic zymodemes. The present result is an advance on our knowledge and corroborates previous findings. However, since the clinical spectrum of the disease may vary and diagnosis based on history may not necessarily be complete, it is suggested that the present result should be taken as tentative until reactivity of the antigens (from different zymodemes) against specifically raised sera in 'animal models' is known.
将溶组织内阿米巴分离株分为四个酶带型，用作间接荧光抗体试验（IFAT）中的抗原；使用从携带不同阿米巴同工酶组的患者中获得的17份血清。发现所采用的培养方法会影响IFAT读数的难易程度。从选定患者（即患有不同形式阿米巴病）获得的血清样本对同源和异源抗原的反应性并未显示出阿米巴酶带型之间存在任何抗原差异。目前的结果是我们知识的进步，并证实了先前的发现。然而，由于该疾病的临床谱可能有所不同，且基于病史的诊断不一定完整，因此建议在了解抗原（来自不同酶带型）对“动物模型”中特异性产生的血清的反应性之前，应将目前的结果视为初步结果。
