Lee K S, Song J Y, Suh M H
Department of Dermatology, Keimyung University School of Medicine, Taegu, Korea.
J Dermatol Sci. 1991 Jul;2(4):316-23. doi: 10.1016/0923-1811(91)90056-4.
The keloid fibroblasts exhibited increased extracellular matrix gene expression, and prominent elevated type I procollagen mRNA when compared to control fibroblasts cultured from the uninvolved skin of normal people. It also showed markedly elevated type I/III procollagen mRNA ratios, but no synthesis of type IV procollagen mRNA by keloid fibroblasts was observed. By in situ hybridization in keloid tissue, high levels of type I and type III procollagen mRNAs were detected in most of the fibroblasts, suggesting the presence of a subpopulation responsible for the increased collagen production. The levels of type I and type III procollagen mRNAs in these fibroblasts were clearly elevated compared to control skin specimens. And concentration of type I procollagen mRNA was found more predominantly than was type III. These results suggest that deposition of collagen in keloid could result from activation of certain fibroblasts responsible for type I procollagen production.
与从正常人未受累皮肤培养的对照成纤维细胞相比，瘢痕疙瘩成纤维细胞表现出细胞外基质基因表达增加，I型前胶原mRNA显著升高。它还显示I/III型前胶原mRNA比率明显升高，但未观察到瘢痕疙瘩成纤维细胞合成IV型前胶原mRNA。通过瘢痕疙瘩组织原位杂交，在大多数成纤维细胞中检测到高水平的I型和III型前胶原mRNA，表明存在负责增加胶原蛋白产生的亚群。与对照皮肤标本相比，这些成纤维细胞中I型和III型前胶原mRNA的水平明显升高。并且发现I型前胶原mRNA的浓度比III型更占优势。这些结果表明，瘢痕疙瘩中胶原蛋白的沉积可能是由负责I型前胶原产生的某些成纤维细胞的激活所致。
