Albano H, Henriques I, Correia A, Hogg T, Teixeira P
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal.
J Appl Microbiol. 2008 Dec;105(6):2187-94. doi: 10.1111/j.1365-2672.2008.03947.x.
This study evaluates the microbial ecology of 'Alheira' by traditional microbiological analysis and a PCR-denaturing gradient gel electrophoresis (DGGE) protocol.
Total microbial DNA from 'Alheiras' was extracted directly from the products and subjected to PCR using Eubacterial primers for 16S rDNA. The amplicons were separated by DGGE. The results demonstrated that different products of the same batch display identical profiles, whereas products from different batches of the same producer could display different DGGE profiles. 'Alheiras' from different producers were distinguishable based on the respective DGGE profiles. The obtained sequences from prevalent phylotypes affiliated with order Lactobacillales and order Bacillales and class Gammaproteobacteria. The same samples were subjected to traditional microbiological analysis. In both methods, lactic acid bacteria were dominant and were present together with other organisms, mainly members of the family Micrococcaceae.
The approach explored in this study allowed the description of the microbial community present in 'Alheira' in particular the diversity of lactic acid bacteria.
This can be useful for the microbiological characterization of traditional products in order to develop new methods of quality control capable of supporting a standardization of the processes, while preserving their typical traits.
本研究通过传统微生物学分析和聚合酶链反应-变性梯度凝胶电泳（DGGE）方法评估“阿尔黑拉香肠”的微生物生态学。
直接从“阿尔黑拉香肠”产品中提取总微生物DNA，使用真细菌16S rDNA引物进行聚合酶链反应。扩增产物通过DGGE分离。结果表明，同一批次的不同产品显示出相同的图谱，而同一生产商不同批次的产品可能显示不同的DGGE图谱。根据各自的DGGE图谱可区分不同生产商的“阿尔黑拉香肠”。从与乳杆菌目、芽孢杆菌目和γ-变形菌纲相关的优势系统发育型中获得了序列。对相同样本进行传统微生物学分析。在两种方法中，乳酸菌均占主导地位，且与其他微生物共同存在，主要是微球菌科的成员。
本研究探索的方法能够描述“阿尔黑拉香肠”中存在的微生物群落，尤其是乳酸菌的多样性。
这对于传统产品的微生物特征描述可能有用，以便开发新的质量控制方法，在保留其典型特征的同时支持生产过程的标准化。
