Deepak Rama Narayana, Crawley Brett, Phang Elaine
Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608, Republic of Singapore.
Trans R Soc Trop Med Hyg. 2008 Dec;102 Suppl 1:S42-4. doi: 10.1016/S0035-9203(08)70012-9.
Sixty unique clinical isolates previously reported as Burkholderia pseudomallei were run in parallel through the API 20NE and VITEK2GN identification systems after 24h growth on Columbia agar with 5% sheep blood. The identifications and confidence levels determined by each test modality were compared. The VITEK2GN identified 47 (78.3%) and the API20NE identified 52 (86.7%) of the isolates as B. pseudomallei. The modal octal profile for the latter was 1156577. The alternative identification most commonly made by both systems was B. cepacia. Comparison of the two modalities gave a Cohen's kappa of 0.6008, suggesting good overall inter-test agreement. Both are good test modalities capable of identifying B. pseudomallei reliably, with the API20NE having the advantage of correctly identifying a slightly greater number of isolates, and the VITEK2 having the advantage of a shorter test turnaround time.
60株先前报告为类鼻疽伯克霍尔德菌的独特临床分离株，在含5%绵羊血的哥伦比亚琼脂上培养24小时后，同时通过API 20NE和VITEK2GN鉴定系统进行检测。比较了每种检测方法所确定的鉴定结果和置信水平。VITEK2GN鉴定出47株（78.3%）分离株为类鼻疽伯克霍尔德菌，API20NE鉴定出52株（86.7%）。后者的标准八进制图谱为1156577。两种系统最常做出的替代鉴定是洋葱伯克霍尔德菌。两种检测方法的比较得出科恩kappa值为0.6008，表明总体检测间一致性良好。两种都是能够可靠鉴定类鼻疽伯克霍尔德菌的良好检测方法，API20NE的优势在于能正确鉴定稍多数量的分离株，而VITEK2的优势在于检测周转时间较短。
