Francis Alex, Aiyar Subramania, Yean Chan Yean, Naing Lin, Ravichandran Manickam
Microbiology Unit, Department of Pathology, Hospital Tengku Ampuan Afzan, 25100 Kuantan, Malaysia.
Diagn Microbiol Infect Dis. 2006 Jun;55(2):95-9. doi: 10.1016/j.diagmicrobio.2005.11.008. Epub 2006 Apr 19.
Isolation and culture of Burkholderia pseudomallei remains the main stay in the diagnosis of melioidosis. Thus, the search for selective and differential media for B. pseudomallei has been ongoing. A number of such media have been reported with varying efficacy. Ashdown medium is the most established selective medium for the isolation of B. pseudomallei. There are no reports of differential media differentiating B. pseudomallei from Burkholderia cepacia. This report documents such a selective and differentiating medium for B. pseudomallei. Of a total of 1042 clinical specimens containing mixed flora and gram-negative isolates that were tested on this medium, 16 of the specimens yielded B. pseudomallei. The isolation rate was found to be 1.5%. This medium was found to be simple and inexpensive, can be made by small laboratories, and called as Francis medium. Based on the colony morphology and color, a preliminary report can be made within 18-24 h for the presence of B. pseudomallei. Our study showed that this medium had an overall sensitivity of 78.4% with a specificity of 92.2%. The use of this medium as an early diagnostic tool will help to reduce mortality and morbidity of melioidosis patients.
伯克霍尔德菌的分离培养仍是类鼻疽诊断的主要手段。因此，寻找用于伯克霍尔德菌的选择性和鉴别性培养基的工作一直在进行。已经报道了许多此类培养基，其功效各不相同。阿什当培养基是用于分离伯克霍尔德菌的最成熟的选择性培养基。尚无关于区分伯克霍尔德菌和洋葱伯克霍尔德菌的鉴别培养基的报道。本报告记录了一种用于伯克霍尔德菌的选择性和鉴别性培养基。在该培养基上测试的总共1042份含有混合菌群和革兰氏阴性菌分离株的临床标本中，有16份标本培养出了伯克霍尔德菌。分离率为1.5%。该培养基简单且成本低廉，小实验室即可配制，被称为弗朗西斯培养基。根据菌落形态和颜色，可在18 - 24小时内初步报告是否存在伯克霍尔德菌。我们的研究表明，该培养基的总体敏感性为78.4%，特异性为92.2%。使用该培养基作为早期诊断工具将有助于降低类鼻疽患者的死亡率和发病率。
