Larina Irina V, Sudheendran Narendran, Ghosn Mohamad, Jiang James, Cable Alex, Larin Kirill V, Dickinson Mary E
Baylor College of Medicine, Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77584, USA.
J Biomed Opt. 2008 Nov-Dec;13(6):060506. doi: 10.1117/1.3046716.
Studying hemodynamic changes during early mammalian embryonic development is critical for further advances in prevention, diagnostics, and treatment of congenital cardiovascular (CV) birth defects and diseases. Doppler optical coherence tomography (OCT) has been shown to provide sensitive measurements of blood flow in avian and amphibian embryos. We combined Doppler swept-source optical coherence tomography (DSS-OCT) and live mouse embryo culture to analyze blood flow dynamics in early embryos. SS-OCT structural imaging was used for the reconstruction of embryo morphology and the orientation of blood vessels, which is required for calculating flow velocity from the Doppler measurements. Spatially and temporally resolved blood flow profiles are presented for the dorsal aorta and a yolk sac vessel in a 9.5-day embryo. We demonstrate that DSS-OCT can be successfully used for structural analysis and spatially and temporally resolved hemodynamic measurements in developing early mammalian embryos.
研究哺乳动物早期胚胎发育过程中的血流动力学变化对于先天性心血管（CV）出生缺陷和疾病的预防、诊断及治疗的进一步进展至关重要。已证明多普勒光学相干断层扫描（OCT）可对鸟类和两栖类胚胎中的血流进行灵敏测量。我们将多普勒扫频源光学相干断层扫描（DSS-OCT）与活小鼠胚胎培养相结合，以分析早期胚胎中的血流动力学。SS-OCT结构成像用于重建胚胎形态和血管方向，这是根据多普勒测量计算流速所必需的。本文展示了9.5天胚胎中背主动脉和卵黄囊血管的时空分辨血流剖面。我们证明DSS-OCT可成功用于发育中的早期哺乳动物胚胎的结构分析以及时空分辨血流动力学测量。
