Whiteman S C, Yang Y, Jones J M, Spiteri M A
Lung Research Group, Institute of Science and Technology in Medicine, University Hospital of North Staffordshire / Keele University, Staffordshire, UK.
Ther Adv Respir Dis. 2008 Feb;2(1):23-31. doi: 10.1177/1753465807087972.
COPD is a common, progressively disabling disease and a major health burden worldwide. Fourier transform infrared (FTIR) spectroscopy provides for sensitive analysis of complex biological samples. COPD pathogenesis involves quantitative and qualitative changes in sputum biosynthesis. This first study explores whether FTIR can produce distinct spectral profiles of human sputum, and capture differences between COPD and health. Sputum obtained from 15 COPD patients and 15 healthy volunteers was analysed using FTIR spectroscopy; differences in peak positions, height and configuration were identified and measured. All samples gave reproducible characteristic IR absorption spectra. The most relevant regions identified were the amide and glycogen rich regions, showing crucial spectral differences between health and COPD relating to peak position shifts or intensity alteration. These novel preliminary findings support further exploration of FTIR sputum profiling in a clinical study to determine its potential as a practical method for monitoring COPD.
慢性阻塞性肺疾病（COPD）是一种常见的、逐渐使人丧失活动能力的疾病，也是全球主要的健康负担。傅里叶变换红外（FTIR）光谱法可对复杂生物样品进行灵敏分析。COPD的发病机制涉及痰液生物合成的定量和定性变化。本第一项研究探讨FTIR是否能产生人类痰液的独特光谱特征，并捕捉COPD患者与健康人之间的差异。使用FTIR光谱法分析了从15名COPD患者和15名健康志愿者获取的痰液；识别并测量了峰位、峰高和峰形的差异。所有样品均给出了可重复的特征红外吸收光谱。确定的最相关区域是富含酰胺和糖原的区域，显示出健康人与COPD患者之间在峰位移动或强度改变方面存在关键的光谱差异。这些新的初步发现支持在临床研究中进一步探索FTIR痰液分析，以确定其作为监测COPD实用方法的潜力。
