Karoubi Golnaz, Gugger Mathias, Schmid Ralph, Dutly André
Division of General Thoracic Surgery, University Hospital Berne, Berne, Switzerland.
Interact Cardiovasc Thorac Surg. 2009 Apr;8(4):393-7. doi: 10.1510/icvts.2008.193995. Epub 2009 Jan 5.
Here we investigate the expression of OCT4 human lung adenocarcinoma and bronchioloalveolar carcinoma (BAC) tumor biopsies and tumor-derived primary cell cultures. OCT4 has been detected in several human tumors suggesting a potentially critical role in tumorigenesis. We assessed the presence of OCT4 in clinical tumor samples of both adenocarcinoma and BAC at the cellular and transcriptional levels, respectively. Furthermore, we evaluated tumor-derived cell cultures for potential differences in OCT4 expression. Immunohistochemical analysis depicted OCT4 in 2 of 8 adenocarcinoma tumor samples and 3 of 5 BAC tumor samples, with no apparent difference in the degree of expression among the sections examined. These results were validated by transcript analysis. Flow cytometric assessment of 11 adenocarcinoma-derived cell cultures and 3 BAC-derived cell cultures revealed significantly higher OCT4 expression in adenocarcinoma tumors compared to their normal counterparts. This, however, was not observed in the BAC cultures. Comparative studies of OCT4 in adenocarcinoma and BAC tumor cell cultures demonstrated a dramatically higher expression in the former. The expression of OCT4 may represent a specific and effective target for therapeutic intervention in adenocarcinoma and BAC. In addition, the aberrant expression and distribution of OCT4 may indicate important parameters concerning the differences between adenocarcinoma and BAC.
在此，我们研究了OCT4在人肺腺癌和细支气管肺泡癌（BAC）肿瘤活检组织及肿瘤来源的原代细胞培养物中的表达情况。OCT4已在多种人类肿瘤中被检测到，提示其在肿瘤发生过程中可能发挥关键作用。我们分别在细胞水平和转录水平评估了腺癌和BAC临床肿瘤样本中OCT4的存在情况。此外，我们还评估了肿瘤来源的细胞培养物中OCT4表达的潜在差异。免疫组织化学分析显示，在8例腺癌肿瘤样本中有2例、5例BAC肿瘤样本中有3例检测到OCT4，在所检查的切片中表达程度无明显差异。这些结果通过转录分析得到验证。对11例腺癌来源的细胞培养物和3例BAC来源的细胞培养物进行流式细胞术评估发现，与正常对应物相比，腺癌肿瘤中OCT4表达显著更高。然而，在BAC培养物中未观察到这种情况。腺癌和BAC肿瘤细胞培养物中OCT4的比较研究表明，前者的表达显著更高。OCT4的表达可能代表腺癌和BAC治疗干预的一个特异性且有效的靶点。此外，OCT4的异常表达和分布可能表明腺癌和BAC之间差异的重要参数。
