Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital of Jiangsu Province, Cancer Institution of Jiangsu Province, Nanjing 210009, PR China.
Oncol Rep. 2011 Jun;25(6):1747-54. doi: 10.3892/or.2011.1252. Epub 2011 Apr 7.
Recent studies demonstrated that microRNA-145 is downregulated in human cancer cells and may function as a tumor suppressor. However, its role in lung cancer tumorigenesis remains unclear. Here, we demonstrated that upregulation of miR-145 reduced the proliferation and invasion as well as the ratio of CD133-positive initiating cells and the tumorosphere growth capacity of the human lung adenocarcinoma A549 cell line. The direct targeting of miR-145 to OCT4 mRNA was predicted by bioinformatic analysis and validated by a luciferase reporter system. We, therefore, confirmed that miR-145 can impair the proliferation of human lung adenocarcinoma-initiating cells by targeting OCT4 and leads to inhibition of lung cancer development.
最近的研究表明，miR-145 在人类癌细胞中下调，可能作为肿瘤抑制因子发挥作用。然而，其在肺癌肿瘤发生中的作用尚不清楚。在这里，我们证明 miR-145 的上调降低了人肺腺癌细胞系 A549 的增殖和侵袭能力，以及 CD133 阳性起始细胞的比例和肿瘤球生长能力。生物信息学分析预测 miR-145 可直接靶向 OCT4 mRNA，并通过荧光素酶报告系统得到验证。因此，我们证实 miR-145 可以通过靶向 OCT4 损害人肺腺癌细胞起始细胞的增殖，从而抑制肺癌的发展。
