果蝇中组织损伤诱导的肠道干细胞分裂
Tissue damage-induced intestinal stem cell division in Drosophila.
作者信息
Amcheslavsky Alla, Jiang Jin, Ip Y Tony
机构信息
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
出版信息
Cell Stem Cell. 2009 Jan 9;4(1):49-61. doi: 10.1016/j.stem.2008.10.016.
Abstract
Stem cell division is essential for tissue integrity during growth, aging, and pathogenic assaults. Adult gastrointestinal tract encounters numerous stimulations, and impaired tissue regeneration may lead to inflammatory diseases and cancer. Intestinal stem cells in adult Drosophila have recently been identified and shown to replenish the various cell types within the midgut. However, it is not known whether these intestinal stem cells can respond to environmental challenges. By feeding dextran sulfate sodium and bleomycin to flies and by expressing apoptotic proteins, we show that Drosophila intestinal stem cells can increase the rate of division in response to tissue damage. Moreover, if tissue damage results in epithelial cell loss, the newly formed enteroblasts can differentiate into mature epithelial cells. By using this newly established system of intestinal stem cell proliferation and tissue regeneration, we find that the insulin receptor signaling pathway is required for intestinal stem cell division.
摘要
干细胞分裂对于生长、衰老和病原体侵袭期间的组织完整性至关重要。成体胃肠道会遇到多种刺激，组织再生受损可能导致炎症性疾病和癌症。成年果蝇的肠道干细胞最近已被鉴定出来，并显示可补充中肠内的各种细胞类型。然而，尚不清楚这些肠道干细胞是否能应对环境挑战。通过给果蝇喂食硫酸葡聚糖钠和博来霉素以及表达凋亡蛋白，我们发现果蝇肠道干细胞能够响应组织损伤而提高分裂速率。此外，如果组织损伤导致上皮细胞丢失，新形成的成肠细胞可分化为成熟的上皮细胞。通过使用这个新建立的肠道干细胞增殖和组织再生系统，我们发现胰岛素受体信号通路是肠道干细胞分裂所必需的。
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