Agale Sonali, Kulkarni Asmita, Ranjekar Prabhakar, Joshi Sadhana
Bharati Vidyapeeth University, Pune, India.
Brain Dev. 2010 Feb;32(2):123-9. doi: 10.1016/j.braindev.2008.12.001. Epub 2009 Jan 6.
There is increasing interest in the role of developmental programming; however, the impact on fetal oxidative stress and brain fatty acid levels has been relatively unexplored. Recent reports have shown that caloric restriction regimens in adult animals reduce the occurrence of chronic diseases by reducing the oxidative stress and altering the long chain polyunsaturated fatty acids (LCPUFA). The present study examined whether caloric restriction during pregnancy alters oxidative stress and essential fatty acid metabolism in mother and offspring at birth. Pregnant female rats were fed either a standard chow (C, n=7) or were calorie restricted (CR, n=7) by feeding 60% of the intake of the control. Oxidative stress marker (malondialdehyde) and polyunsaturated fatty acid profiles in brain and liver were analyzed in both dams and offspring. Total weight gain during pregnancy was lower (p<0.01) in the CR group as compared to the control group but did not affect the litter size and weight. Brain malondialdehyde levels were lower (p<0.05) in dams from the CR group. There was no change in brain and liver LCPUFA levels in both male and female offspring in the CR group. Most of the polyunsaturated fatty acids were reduced (p<0.05) in plasma and brain in the CR dams. Caloric restriction during pregnancy did not alter LCPUFA metabolism in the offspring suggesting that during maternal caloric restriction mothers own stores are mobilized to provide docosahexaenoic acid and arachidonic acid for fetal brain development.
发育编程的作用正受到越来越多的关注；然而，其对胎儿氧化应激和脑脂肪酸水平的影响相对未被充分探索。最近的报告表明，成年动物的热量限制方案通过降低氧化应激和改变长链多不饱和脂肪酸（LCPUFA）来减少慢性疾病的发生。本研究探讨孕期热量限制是否会改变出生时母体和子代的氧化应激及必需脂肪酸代谢。给怀孕的雌性大鼠喂食标准饲料（C组，n = 7）或通过给予对照组摄入量60%的食物进行热量限制（CR组，n = 7）。分析了母鼠和子代脑及肝脏中的氧化应激标志物（丙二醛）和多不饱和脂肪酸谱。与对照组相比，CR组孕期的总体重增加较低（p<0.01），但不影响产仔数和幼仔体重。CR组母鼠的脑丙二醛水平较低（p<0.05）。CR组雄性和雌性子代的脑和肝脏LCPUFA水平均无变化。CR组母鼠血浆和脑中的大多数多不饱和脂肪酸减少（p<0.05）。孕期热量限制并未改变子代的LCPUFA代谢，这表明在母体热量限制期间，母体自身的储备被调动起来，为胎儿脑发育提供二十二碳六烯酸和花生四烯酸。
