Lin Yen-Chih, Brayfield Candace A, Gerlach Joerg C, Rubin J Peter, Marra Kacey G
Division of Plastic Surgery, Department of Surgery, University of Pittsburgh, 1655E Biomedical Science Tower, 200 Lothrop Street, Pittsburgh, PA 15261, USA.
Acta Biomater. 2009 Jun;5(5):1416-24. doi: 10.1016/j.actbio.2008.11.031. Epub 2008 Dec 24.
Polyethersulfone (PES) is a nondegradable, biocompatible, synthetic polymer that is commonly utilized as a membrane material for applications such as hemodialysis, ultrafiltration and bioreactor technology. Various studies have shown surface modification to be a valuable tool in the development of nondegradable materials which promote cell adhesion. Cells of interest include adipose-derived stem cells (ASCs). ASCs are multipotent mesenchymal stem cells that are useful for various regenerative medicine applications. In this study, we hypothesized that PES surfaces modified with a peptide sequence based from fibronectin, such as Arg-Gly-Asp (RGD), Arg-Gly-Asp-Ser and Gly-Arg-Gly-Asp-Ser, would increase ASC adhesion compared to unmodified PES surfaces. The synthetic peptides were covalently bonded to amine-modified PES surfaces using 1-ethyl-3-(dimethylaminopropyl) carbodiimide. The surfaces were characterized using a ninhydrin assay and contact angle measurements. The ninhydrin assay confirmed the presence of amine groups on the surface of peptide-treated PES disks. Advancing water contact angles were analyzed to detect changes in the hydrophilicity of the polymer surfaces, and results indicated our PES membranes had excellent hydrophilicity. The attachment and proliferation of human ASCs was assessed and RGD-treated surfaces resulted in a higher number of attached ASCs after 6 and 48 h, as compared to unmodified PES surfaces. Additionally, varying concentrations of the RGD peptide sequence concentration were examined. These results indicate that PES membranes modified with the RGD peptide sequence can be utilized for enhanced ASC attachment in biomedical applications.
聚醚砜（PES）是一种不可降解的生物相容性合成聚合物，常用于血液透析、超滤和生物反应器技术等应用的膜材料。各种研究表明，表面改性是开发促进细胞粘附的不可降解材料的一种有价值的工具。感兴趣的细胞包括脂肪来源干细胞（ASC）。ASC是多能间充质干细胞，可用于各种再生医学应用。在本研究中，我们假设用基于纤连蛋白的肽序列修饰的PES表面，如精氨酸-甘氨酸-天冬氨酸（RGD）、精氨酸-甘氨酸-天冬氨酸-丝氨酸和甘氨酸-精氨酸-甘氨酸-天冬氨酸-丝氨酸，与未修饰的PES表面相比，将增加ASC的粘附。使用1-乙基-3-（二甲基氨基丙基）碳二亚胺将合成肽共价键合到胺修饰的PES表面。使用茚三酮测定法和接触角测量对表面进行表征。茚三酮测定法证实了肽处理的PES圆盘表面存在胺基。分析前进水接触角以检测聚合物表面亲水性的变化，结果表明我们的PES膜具有优异的亲水性。评估了人ASC的附着和增殖，与未修饰的PES表面相比，RGD处理的表面在6小时和48小时后附着的ASC数量更多。此外，还检查了不同浓度的RGD肽序列浓度。这些结果表明，用RGD肽序列修饰的PES膜可用于生物医学应用中增强ASC的附着。
