Guan T L, Afzelius B A
Institute of Developmental Biology, Academia Sinica, P.R. China.
J Submicrosc Cytol Pathol. 1991 Jul;23(3):351-6.
The bitterling spermatozoon has been examined by electron microscopy using sectioned material and freeze-fracture replicas. Its structure is characterized by a higher degree of order than is seen in spermatozoa of related teleosts. The intramembrane particles in the cell membrane are arranged in a large (around 1 micron in diameter) hexagonal grating pattern at a certain site of the sperm head. The flagellum protrudes from a site slightly posteriorly to this hexagonal grating and is surrounded by a cytoplasmic sleeve that contains regularly spaced filaments in a helical arrangement. The sperm midpiece contains a large mitochondrion enclosed in an elaborate system of membranous cisternae and narrow tubules. Fertilization in the bitterling takes place in the gill chamber of freshwater mussels--a unique brood chamber and site of fertilization for a vertebrate.
通过对切片材料和冷冻断裂复制品进行电子显微镜观察，对麦穗鱼精子进行了研究。其结构的特点是比相关硬骨鱼的精子具有更高程度的有序性。细胞膜中的膜内颗粒在精子头部的特定部位排列成大的（直径约1微米）六边形光栅图案。鞭毛从该六边形光栅稍后方的部位伸出，并被一个细胞质套包围，该细胞质套包含呈螺旋排列的规则间隔的细丝。精子中段包含一个大的线粒体，被一个由膜状池和细管组成的复杂系统所包围。麦穗鱼的受精发生在淡水贻贝的鳃腔中——这是一种独特的育雏室和脊椎动物的受精场所。
