Covelli Laura, Klein Elodie, Gilmer David
Institut de Biologie Moléculaire des Plantes, Laboratoire propre du CNRS (UPR 2357) conventionné avec l'Université Louis Pasteur (Strasbourg 1), 12 rue du général Zimmer, 67084 Strasbourg Cedex, France.
Arch Virol. 2009;154(2):347-51. doi: 10.1007/s00705-008-0306-4. Epub 2009 Jan 11.
The beet necrotic yellow vein virus (BNYVV) RNA-5-encoded p26 protein is involved in the accentuation of symptoms expression of infected Chenopodium quinoa plants and is capable of transcription activation (TA) in yeast. TA was previously localized within the first 55 residues of the p26 protein. Interestingly, TA did not occur when C-terminally deleted forms of p26 were used. We used a genetic screen in the yeast one-hybrid system to select restored TA from randomly generated mutants. The TA domain was found to be located within the first 17 residues. Alanine replacement of aspartic acids 11, 16, and 17 within the full-length p26 prevented TA but did not impair subcellular localization and the symptom expression.
甜菜坏死黄脉病毒（BNYVV）RNA-5编码的p26蛋白参与加重受感染藜麦植株的症状表达，并且能够在酵母中激活转录（TA）。TA先前定位于p26蛋白的前55个残基内。有趣的是，当使用p26的C端缺失形式时，未发生TA。我们在酵母单杂交系统中进行了遗传筛选，以从随机产生的突变体中选择恢复的TA。发现TA结构域位于前17个残基内。全长p26中第11、16和17位天冬氨酸被丙氨酸取代可阻止TA，但不损害亚细胞定位和症状表达。
