Clinical Research Department, Pasteur Institute of Iran, No. 69, Pasteur Ave., Tehran 13164, Iran.
Int J Infect Dis. 2009 Nov;13(6):685-9. doi: 10.1016/j.ijid.2008.10.009. Epub 2009 Jan 9.
Chronic hepatitis B virus (HBV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC). HBV appears to be the most common cause of HCC in Iran. To date, no study has been carried out on the HBV genotype in Iranian HCC patients. This study was undertaken to determine the HBV genotype in Iranian patients with HCC.
Paraffin-embedded tissue samples from 40 patients (31 males and nine females) with HBV-associated HCC were collected from different pathology centers during 2000-2007. Genotyping of HBV was performed by nested PCR-mediated amplification of the target sequence. PCR products were sequenced, and the genotype of each HBV sequence was determined by comparison with sequences of known genotypes in the GenBank. A phylogenetic tree was constructed.
Phylogenetic analysis revealed that all of the HBV isolates were clustered in genotype D.
Our results concur with other reports from Iran, all showing that genotype D is the only detectable genotype in the different clinical forms of HBV infection in this country.
慢性乙型肝炎病毒（HBV）感染是肝细胞癌（HCC）发展的一个主要危险因素。HBV 似乎是伊朗 HCC 的最常见病因。迄今为止，尚未对伊朗 HCC 患者的 HBV 基因型进行研究。本研究旨在确定伊朗 HCC 患者的 HBV 基因型。
在 2000-2007 年间，从不同病理中心收集了 40 例（31 名男性和 9 名女性）HBV 相关 HCC 患者的石蜡包埋组织样本。通过巢式 PCR 介导的目标序列扩增对 HBV 进行基因分型。对 PCR 产物进行测序，并通过与 GenBank 中已知基因型序列进行比较来确定每个 HBV 序列的基因型。构建了系统发育树。
系统发育分析显示，所有 HBV 分离株均聚类在基因型 D 中。
我们的结果与伊朗的其他报告一致，均表明基因型 D 是该国不同临床形式的 HBV 感染中唯一可检测到的基因型。
